桑树皮黄酮的超声波提取及体外抗氧化作用研究

Study on Ultrasonic Wave Extraction and Antioxidant Activities of Flavonoids in Mori Barks

采用超声波提取法对桑树皮中总黄酮进行提取,通过单因素及正交试验确定了桑树皮黄酮超声波提取法的最佳工艺条件:物料粒度大于60目,使用80%乙醇,功率为325W,料液比1:10条件下提取30min,黄酮的得率为7.841‰。利用清除DPPH自由基的能力和还原力作为指标测定了桑树皮黄酮的抗氧化活性。结果表明,桑树皮黄酮有较强的抗氧化能力。超声波提取法与常规热回流提取法的效果及黄酮提取物的抗氧化作用相当。

This investigation adopted ultrasound extract to extract flavonoids in Mori barks. According to the orthogonal test, the optimum extraction conditions are： 60 mesh granularity, 80% ethanol, 325 W ultrasound power, solid/liquid ratio 1：10 and 30 mill processing time with flavonoid yield 7.841%o. The antioxidant activities of flavonoid extracts were evaluated by two antioxidant assays, for 1,1-diphenyl-2-picrylhydrazyl （DPPH） radicals scavenging and reducing power. In comparison with the heat reflux extraction, the results showed that the flavonoid contents and antioxidant activities of extracts obtained by two different extractions are comparable.