高效液相色谱法测定马铃薯薯肉中类胡萝卜素

Determination of Carotenoids in Tuber Flesh by HPLC in Potato(Solanum tuberosum L.)

本实验研究了用高效液相色谱法测定马铃薯薯肉中叶黄素和玉米黄素。采用丙酮:石油醚(1:1,含0.1%BHT)混合液直接提取,用ZOBAXC18(4.6×250mm,5μm)色谱柱,乙腈:甲醇:二氯甲烷(75:20:5,含0.1%BHT)为流动相,进样量为5μl,流速为1ml/min,柱温为25℃,在450nm下检测类胡萝卜素。研究结果表明,该法可以快速、准确地分离测定马铃薯薯肉中叶黄素和玉米黄素的含量,具有良好的线性(相关系数分别为0.9997和0.9987)和重复性,叶黄素和玉米黄素回收率分别达到92.87%和95.68%。而且本方法可有效防止类胡萝卜素氧化,因而非常适用于大批量样品的分析检测。

A high performance liquid chromatographic method was used to determine carotenoids in potato tuber flesh. Carotenoids were extracted from tuber flesh with acetone and petroleum ether（1：1, contain 0.1% BHT）. Using ZOBAX C18 column （4.6×250 mm, 5 ta m）, acetonitrile：methanol：methylene chloride （75：20：5, contain 0.1% BHT） as mobile phase, carotenoids were eluted and monitored at 450 nm.The method showed good linearity （the correlation coefficient were 0.9997 and 0.9987） and repeatability.The recoveries of lutein and zeaxanthin were 92.87 % and 95.68 %, and oxidation of carotenoids was effectively prevented.It proved to be a very useful method for testing large quantities of samples.