阿莫西林在牛肉中残留的高效液相色谱检测及分析

Determination and Analys of Amoxicillin Residue in Beeves with RP-HPLC

建立了反相高效液相色谱分析牛肉样品中阿莫西林残留量的方法。对动物组织样品采用磷酸盐缓冲溶液提取、三氯乙酸沉淀蛋白、固相萃取净化和富集。研究了样品在C18柱上的保留行为和阿莫西林的特征谱图。待测物用高效液相色谱法分离,外标定法定量分析,标准曲线的线性良好,相关系数为0.99934,线性范围为5.0～500.0μg/L。动物组织样品中阿莫西林的加标回收率为77.3%～97.4%,相对标准偏差为1.2%～13.5%,最低检出限低于10μg/kg。该分析方法具有良好的重现性。

A reversed-phase high performance liquid chromatographic method （RP-HPLC） has been developed for the determination of amoxicillin residue in beeves. Beeves were extracted with phosphate buffer, precipitated the proteins with trichioroacetic acid （TCA） solution, and purified and enriched by solid phase extraction （SPE）. The retention results on a C18 column and the chromatography conditions of amoxiciUin were investigated, The samples were separated by RP-HPLC and quantified with external standard calibration curves.There are good linear correlations with the correlation coefficient as 0.99934 between the peak areas and concentrations of amoxicillin .The dynamic linear range is 5.0-500 μg/ml. The average recoveries of amoxicillin in spiked animal tissues range from 77.3 % to 97.4 %, and the relative standard deviations are between 1.2% and 13.5%. The limit of detection is below 10 μg/kg. The relative standard deviations of intra-assay and inter-assay are both within 10%. Tiffs analysis method shows good recurrence.