牛乳铁蛋白对幽门螺杆菌感染大鼠的治疗实验

Therapeutic Experiment of Bovine Lactoferrin on Helicobacter pylori(HP)-Infected Rats

目的观察牛乳铁蛋白（bLf）对感染HP的Wistar大鼠的治疗作用。方法建立Wistar大鼠脾虚动物模型后，空腹灌胃1×10^9efu·mL^-1的Hp混悬液，隔日一次，共三次。于造模后第17天给予bLf进行治疗，并以丽珠得乐和安必仙（抗生素氨苄西林）作为阳性对照药物。结果根据Hp培养、快速尿素酶试验和W—S银染三种检测方法对幽门螺杆菌的检出率，将bLf治疗组和对照组进行比较，不用药的感染对照组Hp检出率在84％-96％范围，丽珠得乐和安必仙二阳性药物对照组Hp检出率在58％～76％和40％-50％范围，bLf治疗高剂量组和中剂量组Hp检出率分别在30％-40％和30％～50％范围，此结果与不用药的对照组比较检出率显著降低，结果显示bLf高、中剂量10d治疗有较好的清除Hp作用。结论bLf在一定剂量下对感染大鼠胃中Hp具有抑制作用。

OBJECTIVE To observe the effect of bovine laetoferrin （blf） on the treatment of HP-infeeted Wistar rats. ME- THODS After establishment of animal model of Wistar rats with spleen deficiency, 1 × 10^9efu · mL^-1 HP suspension was administered by oral gavages every two days（totally 3 times）. The animal model was treated with blf after the model had been set up for 17 days. Meanwhile, Lizhudele and Amoxicilin Capsules were set as positive controls. RESULTS According to positive rate of HP detected by totally 3 methods including HP incubation, rapid urease test and W-S silver-staining, the results of treatment group were compared with these of control group. The results showed that the positive rates of HP for infection-control group with no blf treatment, Lizhudele treatment and Amoxicilin Capsules treatment were within 84% - 96% , 58% - 76% and 40% - 50% , respectively. The positive rates of HP in blf treatment group with middle and high dose were 30% - 40% and 30% - 50% , respectively. Compared with infection-control group with no blf treatment, the positive rate of HP was reduced remarkably and this meant that blf treatment with both middle and high dose for 10 days had remarkable effect on removing HP in gastric mucosal of the rats. CONCLUSION Blf treatment with certain level dose has effect on inhibiting HP-infection in gastric mucosal of Wistar rats.