吡哌酸锌对小鼠烫伤感染的体表保护作用

Protective effect of zinc pipemidate on mouse skin infection after scald

目的观察吡哌酸锌软膏对烫伤后常见的感染菌绿脓肝菌、金黄色葡萄球菌的保护作用，并与阳性对照药磺胺嘧啶银霜、磺胺嘧啶锌软膏进行对比。方法取18～22克小鼠，在麻醉下将其尾部造成烫伤，然后再分别将其尾部没入绿脓肝菌和金黄色葡萄球菌液内，使其感染，尾部套上塑料管，将不同浓度的吡哌酸锌，磺胺嘧啶锌软膏及磺胺嘧啶银霜注入管内，封闭，连续观察14d内小鼠死亡现象拼进行显著性检验；结果综合计算法计算各药ED50为：（1）金黄色葡萄球菌，吡哌酸锌软膏ED50=69．84±18．27mg·kg－1，磺胺嘧啶银霜ED50=73．49±19．98mg·kg。（2）绿脓杆菌，吡哌酸锌软ED50=58．16±15.22mg·kg－1，磺胺嘧啶银霜ED50=80．1±2.24mg·kg－1。磺胺嘧啶锌软膏浓度低于0．4％即有明显抗菌作用。结论吡哌酸锋软膏、磺胺嘧啶银霜、磺胺嘧啶软膏均有明显抗菌作用，前两者相比没有显著差异，但吡哌酸锌软膏的抗菌作用比磺胺胺嘧啶锌强。

To investigate the protective effect of zine pipemidate on infected mouse skin with scald.Methods To establish mouse tail scald model and infect the mice with staphylococcus aureus,pesudomonas aeruginosa. Various concentrated zinc pipemidate paste, silver sulfadiazine cream or zinc sulfadiazine paste was administrated topically to the burn surface, and then, death numbers of the animal were recorded at day 14.Results Death numbers of burn mice after administration of zinc pipemidate paste, silver sulfadiazine cream or zinc sulfadiazine paste were sharply decreased in compared with control group.Conclusions The zine pipemidate paste, silver sulfadiazine cream or zinc sulfaulazine paste are able to control skin infection effectively. There is no significant difference of anti-infective ability between zlnc pipemidate paste and silver sulfadiazine ceram, but zine pipemidate paste is more effective than zinc sulfadiazine paste.