微囊化罗非鱼肝细胞移植治疗大鼠急性肝功能衰竭

Microencapsulated tilapia hepatocytes transplantation to treat acute liver function failure in rats

目的观察微囊化罗非鱼肝细胞移植对急性肝衰大鼠的治疗作用。方法D-氨基半乳糖制备的肝衰大鼠随机分为4组：微囊化组，裸肝细胞组、空微囊组及生理盐水组，腹腔内分别植入微囊化罗非鱼肝细胞、裸肝细胞、空微囊及生理盐水。比较各组间1周死亡率。移植后动态检测各组大鼠总胆红素，比较其差异，动态观察移植物的病理变化。结果移植后1周内微囊化组的存活率较高（57．9％），与其他各组比较差异有统计学意义（P〈0．05）。移植后48h，微囊化组总胆红素（6．21±0．86）μmol／L明显较低，与其他各组差异有统计学意义（P〈0．01）。移植后1周，微囊化组可以收集到形态完整，没有粘连的微囊，其内的肝细胞尚部分保持存活。结论微囊化罗非鱼肝细胞移植能改善肝衰大鼠的肝功能、提高存活率。

Objective To observe the curative effect of microencapsulated tilapia hepatocytes graft to rats with acute liver function failure. Methods All the acute liver failure models in rats were induced by D-galactosamine and divided into 4 groups： microencapsulated tilapia hepatocyte group ,free hepatocyte group, free capsule group, NS group. The one-week survival rate was compared between groups. After transplantation, the changes of TBIL in all groups were dynamically monitored, and the pathological changes of grafts were observed. Results The survival rate （57.9%） in microencapsulated tilapia hepatocyte group was higher than the other groups one week after transplantation （ P 〈 0.05 ）. TBIL （ 6.21 ± 0.86 μmol/L） in microencapsulated tilapia hepatocyte group was reduced markedly as compared with other groups at 48 h after transplantation （P 〈 0.01 ）. One week later, the microcapsules collected from the survival rats remained smooth and intact, and the hepatocytes inside remained alive partially. Conclusion Microencapsulated tilapia hepatocyte transplantation can improve the survival rate of rats with acute liver failure and the liver biochemical function as well.