臭氧氧化预处理对大鼠肾缺血再灌注损伤的热休克蛋白表达的影响

Effects of ozone oxidative preconditioning on HSPTO expression in renal ischemia/reperfusion injury

目的探讨臭氧氧化预处理通过诱导热休克蛋白70（HSP70）的合成，保护大鼠。肾脏缺血再灌注损伤的作用与机制。方法建立原位大鼠单侧。肾缺血再灌注动物模型，I／R前15d经直肠吹入氧气和臭氧的混合气体5．0—5．5ml（臭氧浓度50mg／L，1mg／kg体重，每日1次）。全自动生化分析仪检测尿素氮（BUN）、肌酐（Cr），比色法测定血清的脂质过氧化产物丙二醛（MDA）、超氧化物歧化酶（SOD）。Western blot检测HSP70蛋白的含量；逆转录-聚合酶链反应（RT—PCR）方法检测HSP70的表达。结果肾缺血再灌注24h后，血清中BUN、Cr、MDA明显增高，肾组织内HSP70表达明显增强（P〈0．05），经臭氧氧化预处理后，血清中的BUN、Cr、MDA均降低，SOD升高；HSP70表达升高更加明显（P〈0．05）。结论臭氧氧化预处理可以诱导大鼠。肾缺血再灌注组织中HSP70表达，减轻大鼠。肾脏缺血再灌注损伤。

Objective To investigate the protective effect of ozone oxidative preconditioning （OzoneOP） on heat shock protein 70 （HSP70） expression in renal ischemia/reperfusion injury （I/R） and to disscuss its protective mechanisms, Methods A rat I/R model was established,the renal OzoneOP was induced by rectal insufflation 1 mg/kg, 15 ozone treatments,one per day,5-5.5 ml at an ozone concentration of 50 mg/L, The parameters of renal function were determined by auto-biochemical analyzer. The concentrations of malondialdehyde （MDA） and superoxide dismutase （SOD） were measured with ehromometry. HSP70 was examined by RT-PCR and Western blot. Results Compared with the sham group, the expression of HSP70, the renal function and MDA were increased significantly in the rats undergoing I/ R procedure. After OzoneOP, the levels of BUN, Cr and MDA were significantly decreased and SOD and HSP70 significantly increased. Conclusion OzoneOP can increase the HSP70 expression and alleviate kidney renal I/R injury in rats.