孔雀石绿与牛血清白蛋白的相互作用

Interaction between Malachite Green and Bovine Serum Albumin

运用荧光光谱和紫外-可见吸收光谱研究了在缓冲溶液中不同温度下孔雀石绿(MG)与牛血清白蛋白(BSA)之间的相互作用.实验结果表明,MG对BSA的内源荧光猝灭为静态猝灭过程.测定了该反应在不同温度下的结合常数KA,KA分别为7.69×104L·mol-1(10℃)、5.31×104L·mol-1(20℃)和4.85×104L·mol-1(37℃),MG与BSA以摩尔比1∶1结合.根据F’rster非辐射能量转移理论,求出了37℃时给体(MG)和受体(BSA)之间能量转移效率和结合距离分别为E=0.1635和r=2.30nm.计算出的热力学参数表明,MG和BSA之间的作用力主要是通过氢键和范德华力相互作用.

The interaction between malachite green （MG） and bovine serum albumin （BSA） was investigated via fluorescence and ultraviolet-visible absorption spectra in buffer solutions at different temperatures. The experimental results showed that static quenching was involved in adding MG in BSA solution. The binding constants （KD were7.69×10^4L·mol^-1（10℃）、5.31×10^4L·mol^-1（20℃）, and 4.85×10^4L·mol^-1（37℃）, respectively, and they reacted at a molar ratio of 1：1. The energy transfer efficiency E was 0.1635 and the binding distance r was 2.30 nm between MG and BSA according to Forster non-radiative energy transfer mechanism. The interaction between MG and BSA was driven mainly by hydrogen bonds and Vander Waals force according to thermodynamic parameters.