同期发情处理方法对受体牛胚胎移植效果影响的研究

Effect of Different Methods of Estrus Synchronization on the Pregnant Rate of Cow's Embryo Transplantation

试验设计3个组,分别采用CIDR+PGF2α法、PGF2α一次注射法和PGF2α二次注射法3种不同的处理方法对120头西门塔尔杂交受体牛进行同期发情处理。结果表明:CIDR+PGF2α法同期发情处理的受体牛黄体不合格率达47.4%,显著高于其他两组(P<0.05);胚胎移植产犊率为45.0%,分别比PGF2α一次注射法和PGF2α二次注射法低10.2和13.1个百分点。PGF2α二次注射法同期发情处理后的受体牛黄体不合格率仅20.5%,低于PGF2α一次注射法(P>0.05),显著低于CIDR+PGF2α法(P<0.05);胚胎移植产犊率达58.1%,均高于其他两组。本试验初步显示:采用PGF2α二次注射法进行同期发情处理受体牛效果好,成本较低,操作方便,产犊率也较高。

In order to study the effect of different methods of estrus synchronization on the pregnant rate of embryo transplantation, 120 Simmental crossed dairy cows were randomly divided into three groups. Group 1, CIDR intravaginal sponges were implanted for 12 days and in the 9th day injection PGF2α 0.6mg. Group 2 were once muscle injected 0.6mg PGF2α during the 8-15th day of estrus period. Group 3 were injected 0.6mg PGF2α twice, with 11-day interval between two administration. Results showed that in group 1, recipients＇ luteal unquanlified rate was 47.4%, significantly higher than that of group 2 and group 3 （P〈0.05）. After embryo transplantation, calving rate of group 1 was 45.0% and decreased 10.2 and 13.1 point of percent, compared with group 2 and group 3 ,respectively.In group 3, after estrus synchronization the luteal unquanlified rate was only 20.5%, lower than group 2（P〉0.05） and significantly lower than group l（P〈0.05）. After embryo transplantation, calving rate of group 3 was 58.1%, was higher than other groups. The results revealed that cows were treated by two injections 11 clays apart of PGF2α showed better effect of estrus synchronization, the method cost lower and operated easily and got higher calving rate.