山葵对肺癌组织hnRNP A_2/B_1表达的影响

Effect of wasabi on the expression of hnRNP A_2/B_1 in lung cancer

目的通过建立大鼠肺癌模型,研究山葵提取液对肺癌早期分子标志物hnRNP A2/B1的调节作用,进一步阐明山葵抗肺癌作用的分子机制。方法将36只Wistar大鼠随机分为肺癌模型组(对照组)和山葵治疗组(实验组)。按1mL碘油内含3-甲基胆蒽(MCA)50mg配制致癌质碘油。每只大鼠均用MCA碘油溶液0.1mL在左肺叶支气管内灌注诱癌。实验组于诱癌前1周开始胃饲山葵提取液至动物被处死,对照组于同一时间胃饲等量生理盐水。造模后第30、60及90d分批处死大鼠,每次6只。免疫组织化学法和RT-PCR法检测肺癌组织hnRNP A2/B1的蛋白和基因表达。结果山葵提取液能降低肺癌组织hnRNP A2/B1的蛋白表达,在第30、60及90d的抑制率分别为51.0%、51.0%和45.1%。山葵提取液同时降低了hnRNP A2/B1 mRNA的表达,在第60和90d的抑制率分别为79.5%和58.0%。结论山葵提取液能降低肺癌早期分子标志物hnRNP A2/B1的表达,可能是其抗肺癌的分子机制之一。

Objective An animal model of lung cancer was established to study whether wasabi could inhibit the expression of hnRNP A2/B1 in lung. Methods Thirty-six Wistar rats were randomly divided as model group and wasabi group. 0.1 mL of arcinogenic iodized oil [50 mg 3-methylcholanthrene （MCA） in 1 mL carcinogenic iodized oil] were instilled intratracheally to induce lung cancer. A week before instillation of MCA, the wasabi group was orally administered wasabi extract solution until the animals were killed while the model group was given isometric saline at the same time. Six rats in each group were randomly killed on 30^th day, 60^th day and 90^th day. Immunohistochemisty and RT-PCR were used to measure the protein and mRNA expression of hnRNP A2/Bl , respectively. Results Wasabi lowered the protein expression of hnRNP A2/B1 with a total inhibitory rate of 48.5%. At the 30^th ,60^th and 90^th day, the inhibitory rate was 51.0%, 51.0% and 45.1% respectively. Meanwhile, wasabi lowered the mRNA expression of bnRNP A2B1 with a total inhibitory rate of 60.5%. At the 60^th and 90^th day, the inhibitory rate was 79.5% and 58.0%, respectively. Conclusion Wasabi solution can down-regulate the expression of hnRNP A2/B1 wbich may be a molecular mechanisms by which wasabi inhibits lung cancer.