中草药对MRSA临床株的抑菌作用研究

A study on bacteriostatic action of Chinese herbal medicine on MRSA clinical strain

[目的]研究中草药对耐甲氧西林金黄色葡萄球菌(MRSA)临床株的抑菌作用,探讨MRSA感染的治疗措施。[方法]按照美国临床实验室标准委员会(NCCLS)推荐的标准,采用苯唑西林-琼脂稀释法与聚合酶链式反应(PCR)检测mecA基因两种方法,鉴定MRSA临床株;将9种中草药制备成一定浓度的水煎液,用二倍微孔稀释法测定对MR-SA临床株的最低抑菌浓度(MIC)。[结果]经鉴定所用的临床分离株为MRSA菌株,且对苯唑西林高度耐药;9种中草药水煎液对MRSA临床株有不同程度的抑制或杀灭作用,其中黄芩、黄连、夏枯草、连翘4种中草药的抗菌效果较好。[结论]清热解毒中草药对MRSA临床株具有一定的抗菌作用。

Objective： To study the bacteriostatic action of Chinese herbal medicine on methicillin- resistant staphylococcus aureus （MRSA） clinical strain, and to probe into therapeutic measures for MRSA infection patients. Methods： According to the recommendation of American National Committee for Clinical Laboratory. Standards （NCCLS）, clinical MRSA strain was identified by both oxacillin-agar dilution and polymerase chain reaction to detect rnccA gene two different methods. Then totally 9 kinds of Chinese medicinal herbs was prepared into certain concentration solution and the minimum inhibitory concentration （MIC） was determined by adopting double micropore dilution method. Results： Through identification, clinical separated strains were surely MRSA with high oxacillin - resistance. Nine Chinese medical herbs solution had bacteriostasis or bactericidal effects on the clinical strains d MRSA to different degree. Among them, radix scutellariae, rhizoma coptidis, spica prunellae and fructus forsythiae had stronger effects. Conclusion; Chinese medicinal herbs of heat-clearing and detoxicating have some inhibition effect on clinical strains of MRSA.