6-羟基多巴胺所致急性帕金森病大鼠模型的复制

Acute Rat Model of Parkinson's Disease Brought by 6-Hydroxydopamine

采用6-羟基多巴胺(6-OHDA)纹状体内注射法制作急性帕金森病大鼠模型,为抗帕金森病药物的快速药效学研究提供新的方法。方法10只大鼠随机分为对照组和模型组。给对照组大鼠脑纹状体内注射等量生理盐水、模型组同一部位注射6-OHDA 4μL(12μg/4μL)制作大鼠多巴胺(DA)能神经元损伤的急性模型,术后第7d进行微透析试验,将透析液注入高效液相一电化学检测器(HPLC-ECD)测定各组纹状体细胞外液中DA、3,4-二羟基苯乙酸(DOPAC)和高香草酸(HVA)的含量。结果与对照组相比,模型组动物纹状体细胞外液中DA、DOPAC和HVA含量均显著性降低,具有统计学差异(P<0.05,P<0.01或P<0.001)。采用6-OHDA纹状体内注射法可以复制急性帕金森病大鼠模型。

To make the acute rat model of Parkinson＇ s disease by using the neurotoxic agent 6-hydroxydopamine. Methods： 10 rats were divided into control and model groups.4μL 6-hydroxydopamine（12μg/4μL） was injected into the right striatum of the model rats and the equal volume of 0.9% saline was given to the same part of the control group. Seven days later, the striatal extracellular fluids were gained by brain microdialysis. The methods of high performance liquid chromatography with electrochemical detection were used to measure the striatal extracellular levels of DA, DOPAC, and HVA. The results showed that the extracellular levels of DA, DOPAC and HVA in the model group were far less than that of the control one （P 〈 0.01 vs model group）. Our findings demonstrated that the injection of the neurotoxic agent 6-hy- droxydopamine into the right striatum of rats could immitate some pathological changes of PD.