栽培小豆种质资源遗传多样性SSR标记分析

Genetic Diversity of Cultivated Adzuki Bean(Vigna angularis) Germplasm Resources Based on SSR Markers

利用7对SSR引物对来自中国、日本、韩国、不丹、越南等5国的104份栽培小豆种质资源基因组DNA进行扩增分析。共获得了44条多态性带,平均6.29条。104个小豆种质材料以遗传相似系数0.28为阀值,聚类分析将104份栽培小豆种质材料分为14大类,结果表明中国西南地区的栽培小豆种质存在较丰富的遗传多样性;初步认为中国西南地区可能是小豆的一个起源中心和遗传多样性中心。同一地域或气候相似地区的材料聚为一类,说明供试材料遗传背景与其原产地背景有一定关联性。

SSR （Simple Sequences Repeats） variation was assessed in 104 sample of adzuki bean from China, Japan, Korea, Bhutan, and Vietnam with 7 pared-primers. The results showed that there was a total of 44 polymorphic bands averaging 6.29 bands per primer. The cluster analysis showed that fourteen groups can be clustered when genetic similarity coefficient was given as 0.28. It appeared that Adzuki bean has extraordinary genetic diversity in southwest China; we primarily believed that the southwest china was a origin and genetic diversity center of Adzuki bean; the cluster has obvious association with the genetic races and different adzuki bean geographical regions.