一株寄生油松毛虫的白僵菌毒素化学成分

Isolation and identification of toxins inhibiting Dentrolimus tabulaeformis from an antagonistic strain of Beauveria

【目的】从一株寄生于油松毛虫(Dentrolimus tabulaeformis Tsai et Liu)越冬幼虫上的高毒力卵孢白僵菌(Beauveria tenella)的发酵液中,分离纯化出对油松毛虫幼虫具有杀虫活性的毒素物质。【方法】对这株白僵菌进行了液体培养,用乙酸乙酯对发酵液进行萃取,然后对粗提物进行了硅胶色谱分离,利用GC/MS对第6组物质进行化学成分分析。【结果】其发酵液的乙酸乙酯粗提物对油松毛虫具有杀虫活性(校正死亡率42.52%)对粗提物进行了硅胶色谱分离,共得到6组物质,其中第6组物质对油松毛虫的校正致死率达80.26%。利用GC/MS对第6组物质进行化学成分分析,得知其包括17个组分,其中相对含量大于10%的有:2-哌啶酮占14.02%,2-香豆满酮占47.10%,六氢化-吡咯环[1,2-a]吡嗪?1,4-二酮占21.05%。用其中的2-哌啶酮和2-香豆满酮分别作杀虫试验,油松毛虫的校正死亡率分别为83.32%和91.61%。【结论】2-哌啶酮和2-香豆满酮是该白僵菌菌株的代谢毒素物质。

[Objective] We used submerged fermentation to cultivate a strain of the entomopathogenic fungus Beauveria tenella isolated from the infected larvae of Dentrolimus tabulaeformis in Pinus tabulaeformis forest in Chengde of Hebei Province in China [Methods] We used ethyl acetate to extract antagonistic components from the fermentation broth and used silica gel column chromatography and GC/MS to separate and identify the components. [Results] Six compounds were obtained by silica gel column chromatography. The sixth compound had higher activity to kill the larvae of Dentrolimus tabulaeformis with a corrected mortality rate of 80%. Seventeen compounds were separated and identified by GC/MS in the 6th group, of which 3compounds were more than 10%, 2-Piperidinone（14.02%）, 2-coumaranone（47.10%）, and Pyrrolo[1,2-a]Pyrazine-1,4-dione, hexahydro （21.05%）. [Conclusion] 2-Piperidinone and 2-coumaranone had insecticidal activity （corrected mortality rate reached 83.32% and 91.61% respectively） and were the most important toxic substances to control pests.