新疆哈萨克族食管鳞癌中人乳头状瘤病毒感染的检测

The Prevalence of HPV Infection in Esophageal Squamous Cell Carcinoma in Kazakh of Xinjiang Province

目的:检测新疆哈萨克族食管鳞癌患者中人乳头状瘤病毒(HPV)DNA的总感染率,通过统计学分析,探讨HPV感染与新疆哈萨克族食管鳞癌的相关性。方法:采用聚合酶链反应(PCR)技术检测了318例新疆哈萨克族食管鳞癌(ESCC)石蜡包埋组织中HPVDNA的感染情况,其中117例用直接裂解法制备样本DNA,201例用酚-氯仿-异戊醇提取DNA;PCR产物双向测序,测序结果应用BLAST在线分析,统计处理采用χ2检验,分析HPV与新疆哈萨克族食管鳞癌发生的相关性。结果:在新疆哈萨克族食管鳞癌组织中,HPVDNA总检出率为64.5%(205/318),其中直接裂解法组HPVDNA检出率为82.9%(97/117),酚-氯仿-异戊醇提取DNA组HPVDNA检出率为53.7%(108/201),二者之间差异有统计学意义(P<0.05)。按照病理分化程度对哈萨克族ESCC样本DNA中HPV总感染率进行分析发现,高分化ESCC中HPV阳性率为63.2%,中分化ESCC中HPV阳性率为69.8%,低分化ESCC中HPV阳性率为50.0%,三者之间差异无统计学意(P>0.05)。结论:HPVDNA感染与新疆哈萨克族食管鳞癌存在相关性,可能是新疆哈萨克族食管癌发生的重要因素之一。

Objective： To evaluate the prevalence of HPV infection in esophageal sqnamous cell earcinoma（ESCC） in Kazakh of Xinjiang province. Methods： Polymerase chain reaction（PCR） was employed to screen HPV iufection in samples from 318 ESCC cases. Using DNA extraction with phenol-Tris/chloroform and crude cell suspensions, we also compared the presence of HPV in the same samples. The correctness of PCR products of HPV DNA were demonstrated hy random-sampled sequencing. The X^2 test was used to evaluate the d iflerence in the distribution of HPV infection. Results： Of the 317 ES-CC samples. 204 （63%） were positive for the HPV LI gene. The posilive rate of HPV LI gene was 53.5% in the group of DNA extraction with phenol-Tris/chloroform, and was 83%（97/117） in the group nf erude cell suspensions, with a signifi- cant difference （P〈0.05）. No significant difference was found in HPV general infection rates among different pathological differentiations. Conclusion： HPV DNA infection is related to ESCC in Kazakh of Xinjiang province. It may play a role in the occurrence and development of ESCC.