MMP-12和TIMP-1在大鼠COPD模型肺组织表达及糖皮质激素对其表达的影响

Expression of MMP-12,TIMP-1 and correlated with glucocorticoids in COPD rat model

目的观察基质金属蛋白酶及其抑制因子在大鼠COPD模型肺组织中的表达以及糖皮质激素对其的影响。方法Wistar大鼠24只随机分为对照组、模型组和布地奈德组。气道内注入LPS结合烟熏的方法建立COPD模型,以支气管肺泡灌洗液内白细胞分类计数和HE切片来评价COPD大鼠模型炎症和肺气肿程度。免疫组化方法检测MMP-12、TIMP-1的表达情况。结果模型组大鼠肺组织内MMP-12、TIMP-1表达及MMP-12/TIMP-1值均明显高于健康对照组(P均<0.05)。布地奈德组与模型组之间MMP-12、TIMP-1表达及其比值均无明显差异(P均>0.05)。结论大鼠COPD模型肺组织中MMP-12及TIMP-1表达升高,并且呈现出蛋白酶优势的状态,而糖皮质激素不能调节MMP-12及TIMP-1的表达。

Objective To investigate the expression of MMP-12, TIMP-1 and the correlation with glucocorticoids in COPD rat model. Methods 24 Wistar rats were divided into control group, model group and budesonide group randomly. Airway inflammation was valuated by BALF. The expression of MMP-12 and TIMP-1 were determined by immunohistochemical method. Results The expression of MMP-12,TIMP-1 and MMP-12/TIMP-1 in model group were higher than those in control group（P〈0.05）, and that between budesonide group and model group had no significant difference （P〉0.05）. Conclusion There is higher expression of MMP-12 and TIMP-1 in COPD rat model and budesonide inhalation of foggy can not regulate their expresslon