益生菌对异硫氰酸萘酯诱导淤疸幼鼠胃肠激素的影响及其干预胆汁淤积的机制

Effect of Probiotics on Gastrointestinal Hormine in Intrahepatic Cholestasis Rats Induced by Alpha-Naphthy-lisothiocyanate and Mechanism of Probiotics on Cholestasis

目的通过观察益生菌制剂双歧三联活菌片对异硫氰酸萘酯(ANIT)诱导的急性肝内胆汁淤积幼鼠胃肠激素的影响,探讨其干预胆汁淤积的可能机制。方法SD幼鼠72只随机分为对照组(8只)、中毒组(32只)、干预组(32只)。适应性喂养3 d后,中毒及干预组大鼠一次性灌服ANIT(200 mg/kg)诱导急性肝内胆汁淤积;干预组于ANIT灌胃前2 d,每天灌服双歧三联活菌片[4.2×108个活菌/(kg.d)]。观察各组在灌服ANIT后48、96、144、192 h各时间点胆汁流量、血总胆红素(TB)、ALT及胃动素(MTL)、血管活性肠肽(VIP)的变化。应用SPSS 12.0软件对资料进行统计学分析。结果1.灌服ANIT后48 h中毒及干预组胆流中断,血清TB和ALT水平明显升高,但干预组较中毒组程度轻。48 h后胆汁流量、TB和ALT逐渐恢复,干预组在144 h恢复正常,中毒组192 h基本恢复。2.ANIT灌胃后48 h,中毒、干预组血浆MTL水平较对照组明显降低,VIP较对照组明显升高,但干预组较中毒组程度轻;48 h后,MTL、VIP逐渐恢复,但干预组较中毒组恢复快。另外,在192 h干预组MTL较对照组升高,VIP较对照组降低。结论双歧三联活菌片能够通过其中的双歧杆菌、乳酸杆菌调节胃肠激素的分泌,从而促进肠道运动,有助于胆汁淤积的恢复。

Objective To investigate the effect and mechanism of probiotic products on alpha -naphthylisothiocyanate（ANIT） induced intrahepatic cholestasis of rats. Methods Seventy - two SD rats were randomly divided into 3 groups ： control group （ n = 8 ）, intoxication group （n = 32） and intervention group（ n = 32）. After 3 days adapted feeding, all rats received a single intragastric administration of ANIT （200 mg/kg） to induce acute intrahepatic cholestasis. Probiotics[4.2 × 10^8/（kg·d）] was given to intervention group 2 days before ANIT was administrated. After ANIT was given, biliary flow, alanine aminotransferase transaminase （ALT）, total bilirubin （TB） and gastrointestinal hormone were recorded in every 48 h till 192 h. SPSS 12.0 software was used to analyze the data. Results 1. Forty - eight hours after ANIT was gavaged, the biliary flow of intoxication and intervention groups were lowered while ALT and TB were increased. But the above observation items of intoxication group were more significantly altered than those of intervention group. Then, they all recovered gradually. Those of intervention group returned to nearly normal levels 144 h after ANIT was gavaged while those of the intoxication group recover at 192 h. 2. After ANIT was gavaged, MTL of intoxication and intervention groups was lowered while vasoactive intestinal peptide（VIP） increased. But the above items of intoxication group were more significantly altered than those of intervention group. Then, they all recovered gradually and the intervention group was much more early to returned to normal levels than the intoxication group at 192 h. Conclusions Probiotics can promote the bowel movement through increasing the secretion of gastrointestinal hormone, then relieve the cholestasis.