TIA-1通过影响肝细胞转录因子的活性调节HBV的e抗原和s抗原表达

TIA-1 may interact with liver-enriched transcription factors and regulate HBV s and e antigen expressions

目的证明TIA-1分子具有调节HBV基因表达的功能。方法TIA-1真核表达质粒pcDNA3-TIA-1,用脂质体转染HepG2.2.15细胞后,ELISA检测e和s抗原的表达;RT-PCR检测与HBV复制有关的转录因子HNF1、HNF2、HNF3、HNF4、RXRα、PPARα、C/EBP的表达。结果与正常的HepG2.2.15细胞相比,转染pcDNA3-TIA-1质粒的HepG2.2.15细胞,其e抗原的表达量明显的增高,而s抗原表达量明显的降低。与正常的HepG2.2.15细胞相比,转染pcDNA3-TIA-1质粒的HepG2.2.15细胞中的HNF1、HNF2、RXRα mRNA含量有明显的下降。结论TIA-1蛋白能通过影响与HBV复制有关的转录因子活性,调节HBV e抗原和s抗原的表达。

Objective To identify TIA-1 function in regulating HBV replication. Methods pcDNA3- TIA-1 eukaryotic expression vector was transfected into HepG 2.2. 15 hepatoma cell line with lipofectamine. HBV e antigen and s antigen was checked with ELISA method. RT-PCR was used to identify expression of some transcription factors that involved in HBV transcription such as HNF1, HNF2, HNF3, HNF4, RXRα, PPARα, and C/EBP. Results Compared with normal HepG 2.2.15 hepatoma cell, the expression of e antigen was obviously high and s antigen was depressed in the HepG 2.2. 15 hepatoma cell transfected with pcDNA3-TIA-1 vector. Compared with normal HepG 2.2.15 hepatoma cell, the mRNA level of HNF1, HNF2, RXRα changed significantly. Conclusion These data show that TIA-1 may interact with liver-enriched transcription factors and regulate HBV e antigen and s antigen expression.