摘要
目的探讨PI3K/AKT/FKHRL1信号转导通路在CXCL16诱导的血管平滑肌增殖中的作用。方法运用细胞计数法及MTT法检测CXCL16对于平滑肌细胞增殖的影响;免疫组化法观察CXCL16对PI3K/AKT/FKHRL1信号转导通路的作用,同时观察PI3K/AKT抑制剂LY294002对CXCL16诱导的上述变化的影响。结果CXCL16可明显诱导平滑肌细胞增殖,作用高峰时间在第4天,并且可增加磷酸化AKT及磷酸化FKHRL1的表达。PI3K/AKT阻断剂LY294002可明显抑制CXCL16诱导的平滑肌增殖,同时下调磷酸化AKT及磷酸化FKHRL1的表达。结论CXCL16可能是通过激活平滑肌细胞的AKT通路,诱导AKT及FKHRL1的磷酸化,从而影响平滑肌细胞的增殖。LY294002可以阻断PI3K/AKT/FKHRL1通路而抑制CXCL16诱导的平滑肌细胞增殖。
Objective To study the effec of PI3K/AKT/FKHRL1 signaling in CXCL16 induced Cultured Rat smooth muscle cells proliferation.Methods SMC proliferation checked with Cytometry and MTT,the expression of phospho-AKT and phospho-FKHRL1 with immunohistochemistry,and observe above-mentioned changes incubated with PI3K/AKT inhibitor LY294002.Results Compared with control group,SMC proliferation and of CXCL16 group significantly increased,proliferation apex time at 4 days,also upregulate the expression of phospho-AKT and phospho-FKHRL1.PI3K/AKT inhibitor induce opposite changes.Conclusion CXCL16 can induce SMC proliferation,perhaps via activate AKT and phosphorylation of FKHRL1.LY294002 can inhibit SMC proliferation by block PI3K/AKT/FKHRL1 signal conduction.
出处
《中国实验诊断学》
2008年第4期433-435,共3页
Chinese Journal of Laboratory Diagnosis
