利用组织微阵列技术研究MMP-12在肺癌中的表达及意义

Analysis of MMP-12 Expression and Its Clinical Significance in NSCLC Using Tissue Microarray

目的研究MMP12在人非小细胞肺癌组织中的表达及临床意义。方法应用组织微阵列技术结合免疫组化方法检测120例手术后非小细胞肺癌标本中MMP12的表达状况,并分析其临床病理意义及与预后的关系。结果120例肺癌中MMP12表达阳性率为70%。MMP12表达在患者不同性别、年龄(≥50岁或<50岁)、肿瘤大小(≥3cm或<3cm)、临床分期(I、II期或III、IV期)中差异无统计学意义(P<0.05)。伴淋巴结转移者MMP12阳性表达率(90.4%)高于不伴淋巴结转移者(58.9%),肺鳞癌中MMP12阳性表达率(87.8%)高于腺癌组(31.5%),吸烟者MMP12阳性表达率(86.8%)高于不吸烟者(40.9%),MMP12阳性表达患者生存率低于MMP12阴性表达者;差异均有统计学意义(P<0.05)。结论非小细胞肺癌中MMP12表达与患者性别、年龄、肿瘤大小及肿瘤TNM分期无关;MMP12与肺鳞癌及吸烟关系密切;与淋巴结转移及生存期密切相关;MMP12阳性表达者生存率低、预后差。

Objective To investigate protein expression of MMP12 and its clinical significance in hu- man non small cell lung cancer （NSCLC）. Methods Using tissue microarray combination with immunohistochemical measure to investigate the protein expression status of MMP12 in 120 cases of resected non small cell lung cancer surgical samples, analyzing the relation of expression status of MMP 12 with clinicopathological factors. Resulta MMP12 expression was positive in 84（70%） patients. No significant statistical difference was found in positive expression rate of MMP12 in patients with different gender, age, tumor size and TNM staging （P〉0.05）. Positive expression rate of MMP12 was higher in patients with lymphnode metastasis（90,4%） than in those without lymphnode metastasis （58.9%）, in lung squamas cell cancer （87.8%） than in adenocarcinoma （31.5%）, and it was also higher in smoking patients（86.8%） than non-smoking patients （40.9%）, and the differences were significant （P〈0.05）. The results revealed survival rate of MMP 12 positive patients was lower than that of the negatively expressed.C, onelusion The expression of MMP12 was not associated with agender, age, tumor size or TNM staging, but was closely related with squamas cell lung cancer, smoking, lymphnode metastasis and survival period. Patients with MMP12 expresision had low survival rate and poor prognosis.