烯效唑浸种对大豆种子萌发及保护性酶系的影响

Effect of Seed Soaking with Uniconazole on Germination and Anti-oxidant Enzyme of Soybean

烯效唑是一种延缓型植物生长调节剂,它能够抑制植物体内赤霉素的生物合成、减慢细胞伸长速度。为探讨烯效唑在大豆上的作用效果,以垦农四号大豆为材料,用发芽盒在人工气候箱中进行了大豆种子萌发试验。结果表明:应用25～200mg·mL-1浓度范围的烯效唑浸种可降低大豆发芽势,但对发芽率影响不显著;在种子萌发过程中,随着烯效唑浓度升高,根鲜/干重降低;主/侧根粗增加;根长/体积减小,侧根数减少;在50mg·mL-1浓度范围内,随着烯效唑浓度升高,过氧化物酶、超氧化物歧化酶、过氧化氢酶活性增强,丙二醛含量降低。试验结果进一步证明了烯效唑能够矮化大豆植株生长,提高保护性酶的活性,增强大豆植株的抵抗逆境的能力。

Uniconazole as a kind of growth retardant can inhibit the bio- synthesis of plant gibbereRins in vivio and reduce the elongation speed of plant cell. To discuss the effect of uniconazole on soybean, the soybean seeds of Kennong 4 were soaked with 0,25,50,100,200 mg·L^-1 uniconazole for 6 hours, then germinated in the artificial climate box. Results showed that the soybean germination percentage was decreased while germination potential remained stable when treated by uniconazole with concentration between 25 mg · L ^- 1 and 200 mg· L^ - 1. The weight of fresh/dry root, the diameter of main/ lateral root increased ,while the root length/volume and number of lateral root deereasesd with the increasing of unieonazole concentration in the proceess of seed germination. When the uniconazole concentration was lower than 50 mg · L^-1 , with the uniconazole concentration increasing, the activity of Peroxidase, Superoxide dismutase, Catalase increased, the content of Malondi- adehycle decreased. The result has further proved that uniconazole could inhibit the soybean growth, enhance the antioxidant activity, strengthen the ability of stress tolerance of soybean.