大豆子叶节对潮霉素敏感性研究

Susceptibility of Soybean Cotyledonary Node to Hygromycin B

标记基因的利用是筛选和鉴定转基因植株的有效方法,使用潮霉素能抑制植物的生长。选用3个基因型大豆为材料,以褐化率、分化率为指标,研究了不同质量浓度潮霉素对大豆子叶节分化和丛生芽生根的影响。结果表明:不同基因型大豆对潮霉素的敏感性不同,潮霉素对大豆子叶节适宜筛选浓度黑农35为6mg·mL-1、合丰35和合丰25为4mg·mL-1;丛生芽生根筛选浓度黑农35为2mg·mL-1、合丰35和合丰25为1mg·mL-1。

The use of maker gene is an efficient method to select and identify transgenic plantlets. Hygromycin can destroy the function of ribosome and restrain plantlets growing. The effects of hygromycin on brown rate ,bud differentiation were investigated using 3 soybean varieties. Different mass concentration of hygromycin on the soybean cotyledonary node polarization and bud rooting were studied. The result showed that there was certain difference between the susceptibility of soybean cultivars to selective agents. For cotyledonary node, the optimal mass concentration of hygromycin was 6 mg · L^-l for Heinong 35,4 mg · L^-1 for Hefeng 35 and Hefeng 25. For rooting,the optimal mass concentration of hygromycin was 2 mg· L-l for Heinong 35,1 mg·L^-1 for Hefeng 35 and Hefeng 25.