大豆胞囊线虫白色胞囊与褐色胞囊几种代谢物质比较

Comparative Studies on Metabolites in White and Brown Cyst of Heterodera Glycines Race 4

大豆胞囊线虫成熟后以胞囊的形式存在于土壤中,并由白色逐渐变成褐色。对白色胞囊和褐色胞囊的孵化率以及几种代谢物质(总糖,糖原,海藻糖,甘油,蛋白质,酯酶,海藻糖酶)进行了比较。结果表明:褐色胞囊的孵化率明显比白色胞囊的低;褐色胞囊内的糖原、蛋白质含量较低,酯酶活性较小,但是总糖,海藻糖含量较高,海藻糖酶活性较高,说明褐色胞囊内卵的滞育率较高,其代谢物质中海藻糖和糖原之间可能存在活跃的相互转化;SDS-PAGE蛋白图谱中白色胞囊和褐色胞囊的谱带分别为15和10条,白色胞囊比褐色胞囊多116.6,79.1,68.2,64.3和60.5KDa等5条谱带;酯酶电泳图谱中白色胞囊和褐色胞囊的谱带分别为3和6条,褐色胞囊比白色胞囊多EST0.63、EST0.75、EST0.81等3条谱带。说明新形成的白色胞囊中蛋白质含量较高,在胞囊褐化的过程中,蛋白质含量下降,酯酶同工酶的活性增强。褐色胞囊内卵的孵化率低可能与这些物质变化相关。

Previous research stated that the hatch rate of white cysts was significantly different from that of brown cysts, which indicated that there was different dlapause rate between white and brown cysts. In order to find out the relationships between some metabolites and diapause of Heterodera glycines, hatch rate and metabolites（ total carbohydrate, glycogen, trehalose, glycerol, protein, esterase, trehalase） in white and brown cysts of Heterodera glycines race 4 were studied. The results suggested that hatch rate of brown cyst was lower significantly than that of white cyst. Content of glycogen and protein and activity of esterase in brown cyst were lower than that of white cyst, content of total carbohydrate and trehalose and activity of trehalase in brown cyst was higher. Therefore, diapause rate of brown cysts was higher than that of white cysts, and active mutual transformation between trehalose and glycogen existed in brown cysts. SDS- PAGE pattern showed that there are 15 and 10 protein bands in white and brown cysts respectively, and molecular weight 116.6,79.1,68.2,64.3 and 60.5 KDa proteins existed in white cysts,and without in brown cysts. There were 3 and 6 bands in patterns of esterase isoenzyme in white and brown cysts respectively, EST^0.63, EST^0.75 and EST^0.81 of esterase isoenzyme occurred in brown cysts, and not in white cysts. The result suggested that content of protein was declined, and activity of esterase isoenzyme was enhanced during the white cyst change to the brown cyst. And deduced that low hatch rate of eggs in brown cysts might be related to those metabolites.