摘要
目的探讨身材矮小儿童青少年血浆胃促生长素水平及胃促生长素在生长激素释放激素-生长激素(GHRH-GH)轴中的作用。方法抽取矮小症患儿157例。男115例,女42例;年龄3岁7个月-15岁1个月。取空腹静脉血10mL,分离出血浆标本,通过二种生长激素(GH)激发试验,将其分为3组,生长激素缺乏(CGHD)组37例,生长激素部分缺乏(PGHD)组52例,特发性矮小(ISS)组68例;在健康体检儿童中抽取20例年龄性别相匹配身高正常儿童为健康对照组。采用放射免疫法测定各组血浆中胃促生长素水平。采用ELISA法测定其血胰岛素样生长因子-1(IGF-1)水平。采用化学发光法测定其血清GH水平。分别测定其血睾酮、雌二醇、促黄体生成激素、促卵泡生成激素(FSH)水平。采用SPSS11.5软件对数据进行相关性分析。结果CGHD组患儿IGF-1水平明显低于其他3组,CGHD组患儿空腹血浆胃促生长素水平明显低于ISS组及健康对照组(Pa〈0.05),但与PGHD组无明显差异(P〉0.05)。胃促生长素水平与激发试验中GH峰值呈正相关(r=0.176P〈0.05),与FSH水平呈负相关(r=-0.163P〈0.05),而与其他因素无显著性相关(P〉0.05)。结论胃促生长素在GH分泌中起重要作用,其缺乏可能是下丘脑异常引起GH缺乏的原因之一。
Objective To explore the plasma ghrelin levels in children and adolescents with short stature and the role of ghrelin in growth hormone - releasing honaaone - growth hormone ( GHRH - GH) axis. Methods One hundred and fifty - seven children ( 115 male ,42 female) with short stature were selected. Fasting plasma sample was extracted fi'om 10 mL vemous blood of the children with short stature. Insulin tolerance test and arginine stimulation test was perfonaaed initially to differential diagnosis. And blood samples was divided into 3 categories:37 cases of complete growth hormone deficiency (CGHD) ,52 cases of partial growth hornone deficiency(PGHD) and 68 cases of idiopathic short stature(ISS) during these two growth hormone(GH) provocative tests. Controls consisted of age and gender-match 20 health children. Plasma ghrelin levels were measured by radioimmunoassay. Serum GH was detected by chemiluminescence method, and serum insulin - like growth factor - 1 ( IGF = 1 ) was measured by using enzyme linked immunosorbent assay. Fasting glucose, insulin, testosterone, estradiol ,luteinizing hormone ,follicle- stimulating hormone were measured. Statistical analysis were conducted by using SPSS 11.5 software. Resuits The fasting ghrelin levels of CGHD group Were significantly lower daan that of ISS group and control group(P 〈0.05 ) ,but had no difference with that of PGHD group ( P 〉 0.05 ). The ghrelin levels were positive correlated with the stimulated GH peak ( r = 0. 176 P 〈 0. 05 ), and were inversely correlated with FSH ( r = - 0. 163 P 〈 0.05 ). No other significant correlation was found( P 〉 0.05 ). Conclusion Ghrelin has an important role on GH secretion and abnormal secretion of ghrelin might be a reason of growth hormone deficiency which due to hypothalamic abnormality.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2008年第8期588-589,592,共3页
Journal of Applied Clinical Pediatrics
基金
浙江省卫生厅医药卫生优秀青年科技人才专项基金项目资助(2006QN017)
关键词
胃促生长素
矮小症
生长激素缺乏
特发性矮小
儿童
ghrelin
short stature
growth hormaone deficiency
idiopathic short stature
child