质量放大压电免疫传感器法测定人血清胸苷激酶的临床实验

Detection of Human Thymidine Kinase by Amplified Mass Immunosensor in Clinical Research

目的探讨将质量放大压电免疫传感器方法检测血清中胸苷激酶(TK)作为肿瘤临床诊断的可行性。方法采用质量放大压电免疫传感器方法检测正常人、不同肿瘤患者血清。结果肝癌血清的TK水平最高(2.44±0.46)ng/ml,其次为乳腺癌(1.16±0.24)ng/ml,两者的TK水平均明显高于其他恶性肿瘤(P<0.001)。肺癌、胃肠恶性肿瘤、生殖系统恶性肿瘤以及其他恶性肿瘤血清间的TK水平接近,差异无统计学意义(P>0.05),但与良性肿瘤之间的差异有统计学意义(P<0.001)。此外,良性肿瘤血清的频移值亦显著大于正常人血清(P<0.001)。结论质量放大TK压电免疫传感器法作为一种简便灵敏hTK测定新方法,可用于临床肿瘤普查以及区分肿瘤类型,尤其是肝癌、乳腺癌临床的诊断。

Objective To explore the possibility of sensing detection of thymidine kinase （TK） in sera as a clinical diagnostic indicator of tumor. Methods To detect the TK levels of different tumor serum samples by using amplified mass piezoelectric quartz crystal immunosensors. Results Liver cancer sera have the highest TK level （2. 44± （0. 46 ng/mL）, the galactophore sera take second place （1.16 ± 0. 24 ng/ mL）, and the TK levels of both cancer sera are distinctly different from that of the other malignant tumors（P〈0.001）. The TK levels of lung cancer sera, digestive system cancer sera, Genital system cancer sera and other malignant tumor sera are closely consistent and there is no distinct difference among them （P〉0. 05）, but there are distinctly different from that of the carcinoid sera （P〈0. 001）. Moreover, the TK level of carcinoid sera is distinctly higher than that of normal human sera （P〈0. 001）. Conclusion The amplified mass piezoelectric quartz crystal immunosensors as a new sensitive quantitative method for TK in human sera has great potential for the clinical diagnosis of cancers and the distinguish of cancer types, especially for the clinic diagnoses of liver cancer and galactophore.