摘要
从4种大孔吸附树脂中筛选出HPD-400作为去除无梗五加果粗多糖中蛋白的树脂。考察上样量和洗脱剂用量对树脂去蛋白效率的影响。结果表明,该方法对无梗五加果粗多糖中的蛋白具有较高的去除效率,蛋白去除率达到89.37%,纯化后产品中多糖含量由原来的20.5%提高到83%。
To deproteinize from crude polysaccharide of Acanthopanax sessiliflorus Seem Fruit, HPD-400 was selected from four kinds of macroporous adsorption resins. The influence factors, such as volume of sample and elution volume was investigated. The results show： deproteinization from crude polysaccharide of Acanthopanax sessiliflorus Seem Fruit by macroporous adsorption resin is feasible method. Deproteinization rate is up to 89.37 %, the content of polysaccharide is increased from 20.5 % to 83 %.
出处
《食品研究与开发》
CAS
北大核心
2008年第5期43-46,共4页
Food Research and Development
关键词
大孔吸附树脂
无梗五加
多糖
去蛋白
macroporous adsorption resin
Acanthopanax sessiliflorus seem
polysaccharide
deproteinization
