摘要
首先利用离心的方法获得了瑞士乳杆菌TS206菌体,作出了600nm下瑞士乳杆菌TS206的菌落数和OD值关系曲线,然后利用不同的菌浓组(107、108、109、1010、1011cfu/mL)以及生理盐水对照组和大肠杆菌对照组(109cfu/mL)灌喂0.2mL,分别在第3、5、7、10、15天利用放射性免疫法(RIA)检测小鼠肠粘膜中SIgA含量。结果发现,高剂量组(109、1010组)的在中期SIgA含量开始有所升高但是到后期下降(P>0.05),和对照组没有显著性差异;其余各组与对照组相比P<0.05,故存在显著性差异,所以灌喂瑞士乳杆菌TS206对于SIgA含量的影响是显著的,在一定程度上反映了瑞士乳杆菌对小鼠肠道粘膜免疫的调节作用。实验同时得出最适剂量为108cfu/mL的菌悬液灌喂0.2mL和最佳耐受时间为10d。
In this experiment we used the method of centrifugation to obtain Lactobacillus helveticus TS206, and then made carve between bacteria number and OD value with A600. Adjusted different density of bacteria （10^7, 10^8, 10^9, 10^10, 10^11 cfu/mL） and control group with saline or E.coli （10^9 cfu/mL）instead, they received 0.2 mL, also the normal group mice fed like before. Killed the mice at 3, 5, 7, 10, 15 d at the same time and used radiate immunoassay （RIA） to determinate SIgA in the gut. The correlation analysis showed that there was significant correlation between SIgA and the density of bacteria, high dose（10^9,10^10 group） were little higher than normal and low dose to improve content of SIgA at metaphage but decrease at anaphase（P〉0.05）, had no difference compared with control group. Other groups compared with control groups were all P〈0.05, so they were had notable difference. We have a conclusion is that take Lactobacillus helveticus orally can improve content of SIgA, by some means it reflects that Lactobacillus helveticus TS206 is able to improve mice＇s intestinal mucosa immune. With this study we find the appropriate dose is 0.2 mL（10^8 cfu/mL） and the best tolerance time is 10 days.
出处
《食品研究与开发》
CAS
北大核心
2008年第5期50-53,共4页
Food Research and Development
