高龋患者与无龋健康人口内变形链球菌蛋白表达差异的初步分析

Proteins Differences between Streptococcus Mutans Stains Isolated from Caries-active and Caries-free Individuals by SDS-PAGE

目的:利用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)技术,对来自高龋患者和无龋健康人口内变形链球菌临床株的蛋白组进行初步比较,分析二者蛋白表达的差异,以期进一步明确该菌菌株间致龋性存在差异的原因。方法:采用SDS-PAGE技术,比较分离自高龋患者的变形链球菌593号菌株和无龋健康人的变形链球菌18号菌株的菌体和菌外可溶性蛋白表达的差异。结果:菌体可溶性蛋白电泳图中见593号菌株在75 KDa左右存在特异表达的蛋白条带,且二者在85、60、50、40 KDa左右蛋白表达量上存在差异;菌外可溶性蛋白电泳图中见593号菌株在75、40 KDa左右有特异表达的蛋白条带,而18号菌株在50 KDa左右有特异表达的蛋白条带,二者在60 KDa左右蛋白表达量上存在差异。结论:两菌株的菌体和菌外可溶性蛋白电泳图谱中均可见各自特异表达的蛋白条带,且部分相同位置的蛋白条带在蛋白表达量上也存在差异,表明两菌株的蛋白组存在明显差异,该差异可能是导致其致龋力差异的原因之一。

Objective： The differences between proteome of streptococcus mutans strains isolated from caries -active and that of caries - free individuals were compared to investigate the cause of different cariogenic potential of streptococcus mutans strains. Methods： Differences of cellular and extracellular soluble protein expression between high cariogenic potential streptococcus mutans 593 clinical isolate and low cariogenic potential streptococcus mutans 18 clinical isolate were compared by SDS - PAGE. Results ： In cellular soluble protein electrophoresis, specific band about 75 KDa was observed in 593 strain. The differences of protein expression at 85,60,50, 40KDa between 593 strain and 18 strain were observed. In extracellular soluble protein electrophoresis, specific bands at 75 KDa and 40 KDa were observed in 593 strain ; specific band at 50 KDa was observed in 18 strain. The difference of protein expression amount in about 60KDa between 593 strain and 18 strain was observed. Conclusion： Specific protein bands were observed in cellular and extracellular protein electrophoresis for both strains 593 and 18. There were also different protein expression in certain protein bands. It is indicated that a significant difference existed between the proteome of strains 593 and 18. This difference may contribute to the different cariogenic potential of these two strains.