PHEX蛋白在小鼠成牙本质细胞分化过程中的定量表达

Quantitative Expression of PHEX Protein during the Differentiation of Odontoblasts in Mouse

目的:探讨PHEX在小鼠成牙本质细胞分化过程中的定量表达及其可能的生物学作用。方法:制备小鼠牙胚发育各阶段标本,免疫组织化学方法检测PHEX蛋白在小鼠磨牙牙胚发育各时期成牙本质细胞中的表达情况。对成牙本质细胞中的阳性信号进行定量,并进行统计分析。结果:PHEX蛋白主要表达于分化成熟的成牙本质细胞。在时间上,牙胚帽状期(E16)中未分化的牙乳头细胞无明显表达,钟状期(E18)靠近基底膜处的牙乳头细胞有弱阳性表达,硬组织形成期(P5)可见到成熟的成牙本质细胞中有强烈表达;在硬组织形成期(P5)的空间分布上,颈环处未分化的牙乳头细胞中无明显表达,而未来牙尖处分化成熟的成牙本质细胞则有强烈表达。结论:PHEX蛋白表达具有特定的时间-空间分布特征,PHEX参与了小鼠成牙本质细胞的分化过程,可能在牙本质矿化过程发挥重要的作用。

Objective： To study the quantitative expression and possible biological effects of PHEX protein during the differentiation of odontoblasts in mouse. Methods： Tooth germs at different stages were prepared. Immumohistochemistry was selected to examine the expression of PHEX protein in odontoblasts. Positive signals in odontoblasts were measured and analyzed statistically. Results： The results showed that PHEX protein was mainly expressed in the mature odontoblasts. Horally, PHEX expression was hardly observed in the undifferentiated cells of dental papilla during cap stage（E16）. Weak positive signals were found in the dental papilla cells adjunct to basement during the bell satge （E18）. Mature odontoblasts strongly expressed PHEX protein during the stage of dentin and enamel formation（ P5 ）. Spatially, almost negative expression was found in the undifferentiated cells of dental papilla in cervical loop. However, PHEX was highly expressed in mature odontoblasts in the future dental cusp （P5）. Conclusion： Expression of PHEX features temporospatially distributed. PHEX participates the differentiation of odontoblasts and might play an important role in the mineralization of dentin.