调肝理脾方制剂防治小鼠乙醇性肝损伤的实验研究

Empirical study on protective effect of preparations from Tiaogan Lipi prescription on mouse with hepatic injury induced by ethanol

目的:研究不同工艺的调肝理脾方制剂保护小鼠乙醇性肝损伤的程度及其主要作用环节。方法:采用乙醇肝损伤模型小鼠,以复方鳖甲软肝片为阳性对照,观察调肝理脾方三种不同工艺制剂对乙醇性肝损伤模型小鼠保护效应,观察血液天门冬氨酸氨基转移酶(AST),丙氨酸氨基转移酶(ALT),胆固醇(CHOL),甘油三酯(TG)水平,肝组织丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,及肝脏的病理变化。结果:调肝理脾方3种工艺制剂能显著降低ALT、AST、TG、MAD(P<0.01),显著缓解肝脏病理变化。结论:调肝理脾方制剂对乙醇性肝损伤模型小鼠有保护效应,总体疗效评分显示工艺2组为3种工艺制剂中疗效最好的,而该制剂能通过阻止自由基损伤产生与促进局部损伤修复防治乙醇性肝损伤。

Objective： To compare the protective effect of various preparations from Tiaogan Lipi prescription on mouse ethanol hepatic injury. Methods：The hepatic injury model of mice was replicated with ethanol. Comparing with Fufang Biejia Ruan＇gan prescription,three preparations from Tiaogan Lipi prescription were administrated for protecting the hepatic injury. The contents of AST ,ALT, CHOL and TG in serum and the MDA and SOD in liver tissue were tested. The pathological changes of the liver had been evaluated. Results：Three preparations from Tiaogan Lipi reduces the level of AST, ALT, CHOL, TG,lowers the content of MDA（P〈0.01）, relieved the pathological changes significantly. Conclusion： Three preparations from Tiaogan Lipi prescription were administrated for protecting the hepatic injury.Those data with their total score impression suggest that the second preparation is better than the others and the mechanism is located on inhibiting the lipid peroxides generation and promoting local repair.