地龙降压蛋白对自发性高血压大鼠降压作用及其机制的影响

Antihypertensive effect and mechanism of the antihypertensive protein from lumbricus in spontaneously hypertensive rats

目的:初步探讨地龙降压蛋白对自发性高血压大鼠(spontaneously hypertension rat,SHR)的降压作用及机制。方法:选择16周龄雄性SHR 24只随机分为3组:模型组、地龙降压蛋白低剂量组、地龙降压蛋白高剂量组,另纳入8只同龄雄性Wistar大鼠为正常对照组,干预持续28d。采用无创尾动脉套袖法观察地龙降压蛋白对SHR的降压作用;放射免疫分析法测定SHR血浆、肾脏局部醛固酮(aldosterone,Ald)、6-酮-前列腺素-F1α(6-Keto-PGF1α)含量。结果:地龙降压蛋白低、高剂量组均可明显降低SHR的血压,与模型组比较有显著性差异(P<0.05),可明显降低SHR血浆、肾脏Ald的水平(P<0.05);同时显著升高血浆、肾脏局部6-Keto-PGF1α的含量(P<0.05)。结论:地龙降压蛋白对SHR有降压作用,其降压机制可能与降低SHR血浆、肾脏Ald的水平,升高血浆、肾脏局部6-Keto-PGF1α的含量有关。

Objective： To investigate the antihypertensive effect and mechanism of the antihypertensive protein from lumbricus in spontaneously hypertensive rats（SHR）.Methods：Twenty-four male SHR were randomly divided into control group, model group ,low dose group and high dose group, with 8 rats in each group.The mice were continuously given drugs for 28 days .Then the blood pressure ,the levels of Aldosterone （Aid） and 6-Keto-PGF1α in blood and kidney were measured by radioimmunoassay.Results： Compared with model group, antihypertensive protein from lumbricus could depress the blood pressure in SHR significantly（P〈0.05）, reduce the Ald level and increase the 6-Keto-PGF1α level in blood and kidney obviously（P〈0.05）.Conclusion： The antihypertensive protein from lumbricus has a significant antihypertensive effect in SHR. The mechanism may be related to its actions in reducing Ald level and improving 6-Keto-PGF1α level in blood and kidney.