几种因子对邓恩桉组培中不定芽培养影响的研究

Effects of Several Factors on Adventitious Bud Induction of Eucalyptus dunnii

以不同来源的外植体，组培中采用不同的光源及光照强度、培养基加入抗氧化剂等为试验因子，研究它们对邓恩桉组培中不定芽培养所产生的影响。研究结果表明，以邓恩桉伐桩及干基环剥萌芽条作外植体，其组培中初始不定芽的萌动率比用树冠新梢为外植体的高54％～48％。在邓恩桉组培中以采用自然光照而适宜其不定芽的继代培养。每次于800～1000lux弱光照下7～10天后再于2500～3000lux强光照下培养，不定芽生长良好。培养基中加入浓度为10～15mg／L的抗坏血酸Vc和15～20mg／L核黄素VB2，有利于减缓或抑制其不定芽的褐化。不同的邓恩桉优树间，其组培中不定芽的增殖生长有一定的差异，培养25天时5株邓恩桉优树GLD1、GLD2、GLD3、GLD4和GLD6不定芽的增殖系数为2．8～3．4。

Experiments were conducted to study the effect of different explants, light source, light intensity and antioxidant on adventitious bud induction of Eucalyptus dunnii. The experimental results indicated that the bud germination ratio of sprouting branches was higher than that of crown new shoot. Natural illumination was good for the subsequent generation, the light of 800 - 1 000 lux （7 - 10d） , then change to light of 2 500 - 3 000 lux was suitable for the growth of adventitious bud. Vc （ 10 - 15 mg/L） and VB2 （ 15 - 20 mg/L） were helpful for slowing or restraining buds from getting brown. Adventitious buds from different superior plants exhibited differently in growth and multiplication, the multiplication rate of GLD1 , GLD2, GLD3, GLD4 and GLD6 was 2. 8 - 3.4 times in 25d.