摘要
目的:观察银杏叶提取物(extract of Ginkgo biloba,EGB)对大鼠局灶性脑缺血再灌注梗死区胶质纤维酸性蛋白(GFAP)表达的影响。方法:大脑中动脉插线法制作大鼠局灶性脑缺血再灌注模型。观察各时间点大鼠神经功能缺损程度,并应用Metamoph图像分析系统对结果进行分析。结果:EGB药物组神经功能评分较缺血再灌组好(P<0.05),GFAP阳性细胞于脑缺血2h再灌注24h后即已出现,48、72、96h阳性细胞表达量增加,其中以72h为最多,EGB可抑制缺血后GFAP的表达(P<0.05)。结论:局灶性脑缺血再灌注后,可诱导脑组织GFAP表达增强,EGB可抑制脑缺血后星形胶质细胞GFAP的高表达,提示EGB可能对缺血诱导的星形胶质细胞活化具有抑制作用,这可能是EGB抗脑缺血损伤保护神经元作用的机制之一。
Objective ：To observe the effects of the extract of Ginkgo Biloba （EGB） on the expression of glial fibrillary acidic protein （GFAP） of the astrocyte in focal cerebral ischemia/reperfusion injury rats. Methods： A rat middle cerebral ischemia/reperfusion （I/R） injury model was established by using the suture emboli method. The GFAP expression was analyzed by Metamoph analysis system and the neural functional damage in differerent times was observed. Results ：The recovery of the neural functional damage was better in EGB group than that in I/R group. The GFAP expression was found at 24h after reperfusion and at 2h after ischemia,reaching a maximum of 72h in I/R groups. EGB could inhibit high expression of GFAP in EGB groups. Conclusion ：EGB has inhibitory action on activated astrocytes,which may be one of mechanisms to protect neurons.
出处
《黑龙江医药科学》
2008年第2期3-4,共2页
Heilongjiang Medicine and Pharmacy
基金
黑龙江省卫生厅课题:EGB对脑缺血再灌注大鼠SPY-p38
GAP-43表达和突触超微结构的影响。(No.2006-340
佳木斯大学课题:大鼠脑缺血再灌注损伤后神经可塑性的研究及银杏叶提取物干预的影响(No.2007-s-41)
