舒林酸抑制结肠癌细胞株增殖及其转移机制的研究

The mechanisms of growth and metastasis inhibition on colorectal adenoma cells by sulindac

目的研究非选择性COX抑制剂舒林酸对结肠癌细胞株HT-29生长的影响及其参与引起细胞死亡的可能机制。方法采用四甲基偶氮唑蓝（MTT）比色法检测舒林酸对结肠癌细胞增殖的抑制，激光共聚焦显微镜（LSM）及荧光显微镜观察细胞凋亡，流式细胞仪（FCM）分析其对细胞凋亡及细胞周期的影响。结果MTT显示舒林酸能呈剂量和浓度依赖性抑制HT-29的增殖。TUNEL染色荧光显微镜下观察凋亡细胞呈棕褐色，AnnexinV／PI染色后LsM观察凋亡细胞胞膜绿染，胞核红染或呈桔黄色，FCM显示此药促进细胞的凋亡，使处于CO／G1期的细胞比例显著降低。结论舒林酸可抑制结肠癌细胞株HT-29生长，促进其凋亡，其机制可能与其阻止细胞周期的进展有关。

Objective To investigate the effects and mechanisms of sulindac, a non-selective cyclooxygenase （COX） inhibitor, on the proliferation and apoptosis of colorectal cancer cell line HT-29. Methods HT-29 cells were treated with sulindac. MTT assay and flow cytometry were used to measure the proliferation and apoptosis respectively. The laser scanning microscope （LSM） and the fluorescence microscope were used to observe apoptosis of the cells, and the flow cytometry （FCM） analysis was used to observe the cell apoptosis and cell cycle. Results Sulindac inhibited the cells proliferation and induced apoptosis in a dose- and time-dependent manner. With the TUNEL staining and fluorescence microscope, we found that the apoptosis cell became brown. After the Annexin V/PI staining, we observed that the membrane of apoptosis cells became green with LSM; the nucleosidase became red or crocus. FCM showed that sulindac promoted apoptosis of the cells, made the stage of G0/G1 cells significantly reduced. Conclusions Our results showed that sulindac may inhibit the proliferation and induced the apoptosis of colon cancer cell HT-29, and the mechanism may probably be related to cell cycle arrest.