三聚β肽聚乙二醇化后抗肿瘤转移生物活性研究

Effect of the Pegylation of Trimeric β Peptide on It's Anti-metastasis Activity

目的:比较聚乙二醇(PEG)修饰对三聚β肽(β3)抗肿瘤转移活性的影响。方法:采用黏附试验观察β3及其PEG修饰物(β3-PEG)对肝癌细胞株与纤连蛋白(FN)黏附能力的影响;采用人工基底膜胶观察β3和β3-PEG对肿瘤细胞侵袭重组基底膜能力的影响。结果:β3和β3-PEG对肿瘤细胞SMMC-7721和HCCLM6与FN黏附能力均有显著的抑制作用(P<0.05),且呈剂量及时间正相关;PEG修饰可增强β3对2种肿瘤细胞的黏附抑制作用(P<0.05)。β3和β3-PEG对2种肿瘤细胞迁移和侵袭均有显著的抑制作用(P<0.05)。结论:PEG修饰可增强β3抗黏附作用,但对细胞迁移和侵袭的抑制作用无明显影响。

OBJECTIVE： To observe the effect of the polyglycol （PEG） modification of trimeric β peptide（β3） on it＇s anti - metastasis ability. METHODS： Using adhesion test to study the effects of β3 and β3- PEG on the adhesion of tumor cells to FN. Using artificial basal membrane to study the effects of β3 and β3- PEG on the invasion and recombination of basal membrane of tumor cells. RESULTS： Comparing to negative control, β3 and β3-PEG could both inhibit the adhesion of SMMC- 7721 and HCCLM6 tumor cells to FN with time- dependently（P〈 0.05） . The inhibitory effect of pegylated β3 peptide was stronger than that of β3 peptide（P 〈 0.05） . The mobility and invasion of HCCLM6 and SMMC- 7721 tumor cells were inhibited obviously by β3 and β3-PEG（P 〈 0.05）. CONCLUSION： PEG modification could enhance the anti -adhesion effect of β3, but its inhibitory effect on the mobility and invasion of two kinds of tumor cells was not remarkable.