藻酸双酯钠对硝普钠所致神经细胞株PC12细胞损伤的保护作用

Protective Effect of Polysccharide Sulfate on Sodium Nitroprusside-induced Neurotoxicity in PC12 Cells

目的:观察藻酸双酯钠(PSS)对硝普钠所致PC12细胞损伤的保护作用。方法:培养的PC12细胞分为对照组,损伤组,PSS低、中、高浓度(50、100、150μg·mL-1)组和尼莫地平组,应用MTT法测定细胞存活率,以碘化丙啶染色,流式细胞仪定量分析细胞凋亡百分率;测定细胞中乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)、游离钙及半胱天冬酶-3(caspase-3)水平。结果:与损伤组比较,PSS各浓度组都能显著减少细胞死亡率,降低LDH漏出量及MDA生成,提高SOD活性,降低细胞内游离钙含量及caspase-3活性,抑制PC12细胞凋亡。结论:PSS对硝普钠所致PC12细胞损伤具有显著的保护作用,其机制可能与PSS钙拮抗作用及抗脂质过氧化作用有关。

OBJECTIVE： To investigate the protective effect of polysccharide on sodium nitroprusside- induced neurotoxicity in PC12 cells. METHODS： PC12 cells were cultured and divided into control, damage, PSS（ 50,100,150 μg · mL^-1） and nimodipine groups. The cell survival was measured by MTT assay. The apoptosis was measured by flow cytometry. The levels of LDH, MDA, SOD, [Ca^2＋] i and the caspase-3 activity were determined as well. RESULTS： Compared with the damage group, PSS could significantly reduce cell death rate, lower the efflux of LDH and production of MDA, increase SOD activity, lower the activities of intracellular [Ca^2＋] i and caspase-3 activity, and inhibit apoptosis of PC12 cells. CONCLUSION ： The remarkable protective effect of PSS on sodium nitroprusside - induced damage of PC12 cells is possibly related to its antagonistic action on intracellular calcium accumulation and anti- peroxidation of lipid.