ES-生物烯丙菊酯大鼠亚急性吸入毒性实验研究

Subacute inhalation toxicity study on E-sbiothrin in rats

目的通过本试验,明确ES-生物烯丙菊酯原药的大鼠28d吸入毒性效应,取得该农药28d吸入的最大无作用浓度参数。方法48只SD大鼠分为4组,在不同浓度(5.0、20.0和96.5mg/m3)下吸入染毒28d,试验结束时分别对各浓度组的动物进行血液生化、血常规测定、主要脏器系数测定以及病理检查。结果中、高浓度组雌雄合计动物丙氨酸转氨酶(ALT)与对照组相比升高,而天冬氨酸转氨酶(AST)与ALT比降低。高浓度组雄性和雌雄合计动物乳酸脱氢酶(LDH)与对照组相比降低。高浓度组雄性动物红细胞平均血红蛋白浓度(MCHC)与对照组相比降低。中、高浓度组雌性动物肾体比与对照组相比升高。中、高浓度组大鼠与对照组相比较,气管有不同程度的病理改变。高浓度组大鼠肺和脑垂体有轻度病理改变。结论一定浓度的ES-生物烯丙菊酯原药对SD大鼠有一定的毒作用,在SD大鼠的28d亚急性吸入最大无作用浓度为5.0mg/m3(4h/d)。

Objective To observe the toxic effects on treated SD rats during a 28-day inhalation exposure of E-sbiothrin and to provide the no-observed adverse effect level （NOAEL）. Methods SD rats were exposed to different concentrations of E-sbiothrin （5.0,20. 0 and 96. 5 mg/m^3 ） for 28 days. After the treatment, blood biochemistry and blood routine examination were conducted and animal organs were taken to calculate the organ coefficients and to identify pathologic changes. Results Compared with the controls, ALT increased while the ratio of AST/ ALT decreased in the high and medium concentration groups. LDH and MCHC decreased in male rats of high concentration group. The ratio of kidney and body weight was increased in female rats of high and medium concentration groups. It was found that various pathological changes were observed in tracheas, brains and pituitaries in rats of high and medium concentration groups. Conclusion It was concluded that E-sbiothrin showed toxic effects on SD rats at certain concentrations in the 28-day inhalation exposure and the NOAEL was 5.0 mg/m^3 （4 h/d）.