大鼠肝纤维化对TGF-β信号通路的影响

The Response of Rat's Hepatic Fibrosis to TGF-β Signalling

采用CCl4损伤性大鼠肝纤维化模型,观察肝纤维化对TGF-β信号通路的影响。方法20只雄性Wistar大鼠随机分为2组:一组作为正常组(C组,n=6),另外一组为肝纤维化模型组(M组,n=14)。C组,饲普通饲料及自来水,每次腹腔注射2 mL.kg-1橄榄油,M组于实验第1天腹腔内注射50%CCl4(CCl4:橄榄油=1:1)诱导肝纤维化,每次2 mL.kg-1,每周3次,共6周。实验完备后称重、处死,并用分光光度计测定血清中的ALT和AST活性,用RT-PCR技术检测:Ⅰ、III型胶原蛋白、Fibronection、PAI-1和TGF-βmRNA转录水平。结果:模型组与对照组相比较,ALT和AST活性显著升高(p<0.05),Ⅰ、III型胶原蛋白、Fibronection、PAI-1、TGF-β转录水平同样显著升高(p<0.05)。结论:大鼠肝纤维化与TGF-β信号通路有直接的关系,而且在纤维化肝中表达量明显上调。

Basing on the model of rats hepatic fibrosis induced by carbon tetrachloride（ CCl4 ）to investigate the effects of hepatic fibrosis on TGF - β signaling Methods： throughout the study male Wistar rats were used. Animals were randomly divided into two groups including a healthy control group（ n = 6 ）, a model group （ n = 14）. The model groups were injected intraperitoneally （ip） with 50% CC14 （2 mL/kg/2day） for six weeks, to induce rat liver fibrosis, the results were evaluated by detections of the mRNA level of TGF - β, Collagen type, Collagen type Ⅲ, Fibronection, PAI- 1 and TGF - β by semi - quantitative RT - PCR, the level of Glutamate - pyruvate transaminase（ALT） and glutamic -oxalo acetic transaminase （AST）detected by spectrophotometer following the protocol of the Kit. Results： compared with model group, in rats that model, the mRNA level of TGF - β, Fi- bronection, PAI- 1 ,TGF-β, Smad7 and Collagen type 1, Collagen type 3 in liver tissues were significantly increased（ P 〈 0.01 ） ,ALT and AST too. （ P 〈 0.01 ）. Conclusion： there is directed relationship between TGF - β signaling and rats hepatic. At the same time, the expression lever of TGF - β was increased in hepatic fibrosis.