山东省规模化猪场繁殖与呼吸综合征抗体检测与分析

Analysis and detection of serum antibody about porcine reproductive and respiratory syndrome in Shandong pig farm

本研究利用IDEXX公司猪繁殖与呼吸综合征(PRRS)抗体检测试剂盒对山东省不同地区共16个规模化免疫的猪场的601份猪血清,结果弱毒疫苗免疫猪场猪群抗体阳性率达到90.65%,灭活苗免疫猪场猪群抗体阳性率仅达到75.49%。研究结果表明山东省规模化猪场普遍存PRRS感染,对于预防PRRS而言,弱毒疫苗的体液免疫效果要优于灭活疫苗。

In the present study, to assay the infection of porcine reproductive and respiratory syndrome （PRRS） in Shandong,601 serum sample vaccinated from sixteen pig farms and 227 ones non-vaccinated from eight farms were collected,and enzyme-link immunosorbent assay （ELISA） was used to monitor the antibody against to PRRSV of all the blood serum by the diagnose kit of PRRS antibody from the IDEXX company. The result showed that the average positive rate of PRRSV was 90.66% from pigs immunized by live vaccine and 75.49 % from pigs immunized by inactive vaccine. The result of investigation demonstrated that porcine reproductive and respiratory syndrome was ubiquitous in Shandong and attenuated vaccine was better than inactive vaccine about the effect of humoral response. In other hand, it implies that attenuated vaccine was better than inactive vaccine about the effect of immunity.