摘要
应用电镜、DAS-ELISA技术检测烘烤烟丝样品K326-1和K326-2中的病毒。电镜观察到2个样品中均有类似烟草花叶病毒(Tobacco mosaic virus,TMV)的粒子,在K326-2烟丝中还有类似番茄斑萎病毒属(Tospovirus)的病毒粒子存在于细胞壁及细胞质中。2个样品均与TMV抗血清及西瓜银色斑驳病毒(WSMoV)抗血清呈阳性反应。结果表明经烘烤的烟丝中仍存在病毒。
Viruses in the tobacco shred samples K326-1 and K326-2 were detected by electron microscope and DAS-ELISA. The particles of Tobacco mosaic virus like were found in two samples and Tospov/rus-like particles were found in the cell well and. cytoplasm of sample K326 -2. Two samples had positive reaction to the antibodies of Tobacco mosaic virus and Watermelon silver mottle virus. These results showed that tobacco shred still contained viruses.
出处
《西南农业学报》
CSCD
2008年第2期545-547,共3页
Southwest China Journal of Agricultural Sciences
基金
科技部农业科技成果转化资金项目(02EFN2153005600)
