热休克蛋白70、葡萄糖调节蛋白94和IgG在人肺癌组织中的表达

Expression of Hsp70 Grp94 and IgG in human lung carcinoma

目的:探讨热休克蛋白70(Hsp70)、葡萄糖调节蛋白94(Grp94)、IgG在人肺癌组织中的表达及意义。方法:应用免疫组织化学方法结合病理学图像分析系统研究40例肺癌组织中Hsp70、Grp94和IgG的表达。并用免疫荧光双标染色及激光共聚焦技术研究三者的定位关系。结果:人肺癌组织中Hsp70、Grp94、IgG都有高表达,阳性率分别为65%(26/40)、45%(18/40)和82.5%(33/40),Hsp70阳性定位胞核胞质,Grp94和IgG阳性定位主要在胞质,平均光密度值分别为(5.10±0.32)、(3.52±0.35)和(8.12±0.31)。26例Hsp70阳性的肺癌病例中有10例与IgG有共定位关系,18例Grp94与IgG有共定位关系。结论:Hsp70、Grp94和IgG在肺癌组织中高表达,提示三者在肺癌的发生发展中起重要作用;肺癌肿瘤细胞表达的IgG与Hsp70、Grp94分别存在一定共定位关系,其在肿瘤的发展中可能具有协同作用或者在肺癌组织中存在Hsp70-IgG、Grp94-IgG和Hsp70-Grp94-IgG复合物的形式。

AIM： To explore the expression and significance of heat shock protein 70 （ Hsp70）, glucose regulated protein 94 （Grp94） and immunoglobulin G （IgG） in human lung carcinoma. METHODS： The expression of Hsp70, Grp94 and IgG in 40 human lung carcinomas was studied using immunohistochemical technique and image analysis. The localization among Hsp70, Grp94 and IgG was analyzed by double labeling immunofluorescent staining and laser scanning confocal microscopy. RESULTS： Hsp70, Grp94 and IgG in Human lung carcinomas showed high expression. The positive rate of Hsp70, Grp94 and IgG was 65% （26/40）, 45% （ 18/40）, and 82.5% （33/40）, respectively. The average value of optical density was 5.10 ± 0.32, 3.52±0.35, and 8.12 ±0.31, respectively. Hsp70 was localized in nucleus and cellular cytoplasm while Grp94 and IgG were mainly localized in cellular cytoplasm. Ten cases showed Hsp70 was co-localized with IgG and Eighteen cases showed Grp94 was co-localized with IgG. CONCLUSION： High expression of Hsp70, Grp94 and IgG in Human lung caminomas suggested that Hsp70, Grp94 and IgG might play an important role in the development of human lung carcinoma. IgG is co-localized with Hsp70 or Grp94. The study will lay a theorical basis for further research on antitumor immunotherapy.