摘要
目的:制备单克隆抗体(mAb)并初步用于PTD-bcr/abl融合蛋白的研究,为慢性粒细胞白血病的免疫治疗提供实验依据。方法:在大肠杆菌中表达PTD-bcr/abl融合蛋白,用所获得的蛋白免疫BALB/c小鼠,常规收集小鼠脾细胞与Sp2/0细胞融合,筛选杂交瘤细胞株的阳性克隆,并对其进行一系列鉴定。结果:(1)表达PTD-bcr/abl融合蛋白;(2)制备了抗PTD-bcr/abl融合蛋白mAb;(3)用mAb以多种方法检测PTD-bcr/abl融合蛋白,证实此抗体效价高,特异性强。结论:此抗体可用于PTD-bcr/abl融合蛋白的进一步研究。
AIM： To Preparation and characterization the monoclonal antibody （mAb） to PTD-bcr/abl fusion protein. METHODS： The PTD-bcr/abl fusion protein was expressed in E. coli. BALB/c mice were immunized with specific PTD-bcr/abl. The splenic cells of the mice were fused with Sp2/0 mouse myeloma cells. The hybridoma culture supernatants were screened by indirect ELISA. The property of the antibody was identified by Western blot and immunohistochemistry. RESULTS： The PTD-bcr/abl fusion protein and its mAb were obtained, mAb against PTD-bcr/abl displayed strong specifllity and high affinity. CONCLUSION： An anti-PTD-bcr/abl fusion protein mAb with high affinity was abtained, which could be applied to the research of PTD-bcr/abl.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2008年第5期488-490,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
第四军医大学基金资助项目(CX02A023)
