反相高效液相色谱法测定术后排气口服液中大黄素的含量

Determination of emodin by RP-HPLC in Rhubarb oral solution

目的：建立术后排气口服液中大黄素的含量测定方法。方法：采用反相高效液相色谱法，色谱柱Hypersil C18（4．6mm×250mm，10μm）；流动相为甲醇-0．1％磷酸溶液（85：15）；紫外检测波长290nm；流速1ml／min。结果：大黄素在0．8～16μg／ml浓度范围内呈良好的线性关系，r=0．9999；所含大黄素为20．7mg／L，回收率为97．9％，RSD为0．62％。结论：所建方法具有简便、直观和可操作性强的特点，可作为该制剂的质量控制方法。

OBJECTIVE To explore a method for the assay of emodin in Rhubarb oral solution. METHODS Emodin was determined by RP- HPLC in Rhubarb oral solution. The chromatographic column was Hypersil C18 （4.6 mm × 250 mm, 10 μm）. The mobile phase consisted of methanol- 0. 1% phosphoric acid solution （85： 15）. Detective wavelength was 290 nm. The flow rate was 1 ml/min. RESULTS There was a good linear relationship with the concentration range of 0. 8 - 16 μg/ml of emodin （ r = 0. 999 9）. There was 20.7 mg/L emodin in Rhubarb oral solution. The average recover of emodin was 97.9%, the RSD 0.62%. CONCLUSION This method is simple, operated easy. It may be used for quality control of the preparation.