期刊文献+

神经生长因子对拟老化豚鼠听功能的保护 被引量:7

The hearing protection of NGF to mimetic aging guinea pigs
下载PDF
导出
摘要 目的探讨神经生长因子(nerve growth factor,NGF)对拟老化豚鼠内耳的作用和机制。方法选择4月龄纯种豚鼠24只,双耳廓反应灵敏,随机分为3组,各8只。A组(对照组)给生理盐水2ml/kg;B组给5%D-半乳糖300mg/kg;C组分别给5%D-半乳糖300mg/kg和NGF1000U/kg。均腹腔注射,每日2次,连用4周。各组豚鼠实验前后行ABR测试,处死后分别做透射电镜和TUNEL检测。结果C组豚鼠较B组豚鼠ABR阈值明显降低,TUNEL阳性的毛细胞、螺旋神经节细胞明显减少,差异有统计学意义(P<0.05)。结论NGF能够有效保护拟老化豚鼠的听功能。 OBJECTIVE To explore the function and mechanism of the apoptosis in cochlear of mimetic aging guinea pigs treated with NGF. METHODS The twenty-four four-month-old guinea pigs which auricle reflex were sensitive were divided randomly into three groups: A group were injected to abdominal cavity with physiological saline (2 ml/kg) ; B group were injected to abdominal cavity with 5 % D-galactose (300 mg/kg) ; C group were given 5 % D-galactose (300 mg/kg-) and NGF (1000 U/kg) respectively to abdominal cavity. Three groups had been treated two times a day for four consecutive weeks. All the guinea pigs were tested with auditory brainstem response (ABR) before the experiment and twenty-four hours after the last injection. All the guinea pigs were killed by decapitation. The cochlears were removed and fixed in different fixatives. The specimens were treated by a series of special process and the Corti organs were tested with terminal deoxynucleotidyl transmission-mediated dUTP nick-end-labelling (TUNEL) and transmission electron microscopy respectively. RESULTS The ABR threshold of C group was apparently lower than that of B group (P〈 0.05) . The number of the TUNEL positive outer hair cells and spiral ganglion cells in C group was greatly less than that of B group (P〈 0.05). CONCLUSION NGF can improve the auditory function of mimetic aging guinea pigs.
出处 《中国耳鼻咽喉头颈外科》 北大核心 2008年第4期175-177,共3页 Chinese Archives of Otolaryngology-Head and Neck Surgery
关键词 神经生长因子 豚鼠 衰老 毛细胞 细胞凋亡 Nerve Growth Factor Guinea Pigs Aging Hair Cells Apoptosis
  • 相关文献

参考文献8

  • 1[1]Cadenas E,Davies KJ.Mitochondrial free radical generation,oxidative stress,and aging.Free Radic Biol Med,2000,29:222-230.
  • 2[2]Wei H,Li L,Song Q,et al.Behavioural study of the D-galactose induced aging model in C57BL/6J mice.Behav Brain Res,2005,157:245-251.
  • 3孔维佳,胡钰娟,王琼,许黎,王莹,韩月臣,李隽,刘波,孔雯.大鼠内耳拟老化伴线粒体突变模型的建立[J].临床耳鼻咽喉科杂志,2006,20(19):888-890. 被引量:6
  • 4尹志华,董明敏.D-半乳糖制造老年性聋模型方法的改进[J].中国中西医结合耳鼻咽喉科杂志,2004,12(4):173-175. 被引量:7
  • 5[5]Song X,Bao M,Li D,et al.Advanced glycation in D-galactose induced mouse aging model.Mech Aging Dev,1999,108:239-251.
  • 6[6]Shoji F,Miller AL,Mitchell A,et al.Differential protective effects of neurotrophins in the attenuation of noise-induced hair cell loss.HearRes,2000,146:134-142.
  • 7[7]Shah SB,Gladstone HB,Williams H,et al.An extended study:protective effects of nerve growth factor in neomycininduced auditory neural degeneration.Am J Otol,1995,16:310-314.
  • 8[8]Xia Z,Dickens M,Raingeaud J,et al.Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis.Science,1995,270:1326-1331.

二级参考文献20

  • 1孔维佳,汪吉宝,钟乃川.豚鼠耳蜗电图及听觉脑干电位同步记录法的探讨[J].临床耳鼻咽喉科杂志,1989,3(3):148-152. 被引量:19
  • 2孔维佳,韩月臣,王莹,胡钰娟,刘俊,王琼.维生素E和辅酶Q10对大鼠内耳组织线粒体DNA 4834 bp缺失突变的预防作用[J].中华耳鼻咽喉科杂志,2004,39(12):707-711. 被引量:21
  • 3Song X,Bao M, Li D,et al. Advanced glycation in Dgalactose induced mouse aging model. Mech Ageing Dev, 1999,108(3): 239-251.
  • 4Jordens RG,Berry MD,Gillott C,et al. Prolongation of life in an experimental model of aging in Drosophila melanogaster. Neurochem Res, 1999,24 (2): 227-233.
  • 5黄选兆,汪吉宝.实用耳鼻咽喉科学.第1版.北京:人民卫生出版社,2000.1002-1005.
  • 6Arnesen AR. Presbyacusis-loss of neurons in the human cochlea nuclei. J Laryngol Otol, 1982,96(6) : 503-511.
  • 7Willott JF,Parham K,Hunter KP. Response properties of interior colliculus neurons in middle-aged C57BL/6J mice with presbyacusis. Hear Res, 1988,37 ( 1 ) : 15-27.
  • 8Scholtz AW,Kammen-Jolly K,Felder E,et al. Selective aspects of human pathology in high-tone hearing loss of the aging inner ear. Hear Res, 2001,157 (1) : 77-86.
  • 9Fischel-Ghodsian N,Bykhovskaya Y,Taylor K,et al.Temporal bone analysis of patients with presbycusis reveals high frequency of mitochondrial mutations.Hear Res,1997,110:147-154.
  • 10Van Tuyle G C,Gudikote J P,Hurt V R,et al.Multiple,large deletion in rat mitochondrial DNA:evidence for a major hot spot.Mutat Res,1996,349:95-107.

共引文献10

同被引文献52

引证文献7

二级引证文献16

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部