摘要
目的探讨神经生长因子(nerve growth factor,NGF)对拟老化豚鼠内耳的作用和机制。方法选择4月龄纯种豚鼠24只,双耳廓反应灵敏,随机分为3组,各8只。A组(对照组)给生理盐水2ml/kg;B组给5%D-半乳糖300mg/kg;C组分别给5%D-半乳糖300mg/kg和NGF1000U/kg。均腹腔注射,每日2次,连用4周。各组豚鼠实验前后行ABR测试,处死后分别做透射电镜和TUNEL检测。结果C组豚鼠较B组豚鼠ABR阈值明显降低,TUNEL阳性的毛细胞、螺旋神经节细胞明显减少,差异有统计学意义(P<0.05)。结论NGF能够有效保护拟老化豚鼠的听功能。
OBJECTIVE To explore the function and mechanism of the apoptosis in cochlear of mimetic aging guinea pigs treated with NGF. METHODS The twenty-four four-month-old guinea pigs which auricle reflex were sensitive were divided randomly into three groups： A group were injected to abdominal cavity with physiological saline （2 ml/kg） ; B group were injected to abdominal cavity with 5 % D-galactose （300 mg/kg） ; C group were given 5 % D-galactose （300 mg/kg-） and NGF （1000 U/kg） respectively to abdominal cavity. Three groups had been treated two times a day for four consecutive weeks. All the guinea pigs were tested with auditory brainstem response （ABR） before the experiment and twenty-four hours after the last injection. All the guinea pigs were killed by decapitation. The cochlears were removed and fixed in different fixatives. The specimens were treated by a series of special process and the Corti organs were tested with terminal deoxynucleotidyl transmission-mediated dUTP nick-end-labelling （TUNEL） and transmission electron microscopy respectively. RESULTS The ABR threshold of C group was apparently lower than that of B group （P〈 0.05） . The number of the TUNEL positive outer hair cells and spiral ganglion cells in C group was greatly less than that of B group （P〈 0.05）. CONCLUSION NGF can improve the auditory function of mimetic aging guinea pigs.
出处
《中国耳鼻咽喉头颈外科》
北大核心
2008年第4期175-177,共3页
Chinese Archives of Otolaryngology-Head and Neck Surgery
