骨桥蛋白在喉癌组织中的表达

Expression of osteopontin in laryngeal carcinoma

目的探讨骨桥蛋白(osteopontin,OPN)在喉鳞状细胞癌(简称喉癌)中的表达,并分析其与临床病理学特征以及抗原(CD44v6)的关系。方法采用RT-PCR半定量法及免疫组化SP法检测40例喉癌及癌旁相对正常组织标本中OPN的表达,结合喉癌患者的临床病理学特征及CD44v6的表达进行分析。结果①喉癌组织及癌旁相对正常组织中OPN mRNA的表达率分别为90.0%(36例),17.5%(7例),OPN蛋白的表达率分别为77.5%(31例)、10%(4例);②OPN的表达与淋巴结转移情况、临床分期、病理组织学分级有关,与性别、年龄、原发部位无相关性;③OPN和CD44v6的表达呈显著相关。结论OPN在喉癌组织中的表达高于癌旁相对正常组织,可能参与了喉癌的发生、发展和转移,并与CD44v6有协同作用。

OBJECTIVE To study the osteopontin （OPN） expression in laryngeal squamous cell carcinomas and its correlation with clinicopathological tumor features and CD44v6 expression. METHODS Immunohistochemistry and reverse transcription-PCR were used to study the OPN expression in 40 laryngeal squamous cell carcinomas and adjacent tissues. RESULTS （1）The positive rate of OPN mRNA in LSCC and the adjacent tissue was 90.0 % and 17.5 % respectively, and the positive rate of OPN protein was 77.5 % and 10.0 % respectively. （2）The OPN expression levels were significantly correlated with tumor stage, grade and the presence of lymph node, not correlated with age, gender and tumor primary site. （3）The expression of OPN was significantly correlated with the expression of CD44v6. CONCLUSION OPN expression was higher in LSCC than in adjacent tissue, and was paralleled with CD44v6 expression. OPN maybe directly associated with the development of LSCC.