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铜绿假单胞菌感染诱导U937细胞凋亡的研究 被引量：5

Cell apoptosis of U937 induced by Pseudomonas aeruginosa infection
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摘要 目的探讨铜绿假单胞菌(Pseudomonas aeruginosa,PA)感染与人巨噬细胞系U937细胞凋亡的关系。方法以U937细胞作为PA感染的体外细胞模型,使细胞与细菌浓度比分别为1∶10、1∶20、1∶50及1∶100,用荧光染色技术、Annexin V/PI双染流式细胞仪检测细胞凋亡率。结果细胞与细菌比例为1∶10时即可引起部分U937细胞发生凋亡,Hoechst染色法和Annexin-V FITC/PI双染流式细胞仪检测细胞凋亡率分别为(11.67±1.75)%和(11.68±2.58)%,且U937细胞凋亡百分率随PA感染剂量增加而增加。结论铜绿假单胞菌感染可诱导U937细胞凋亡,且呈剂量依赖。 Objective To investigate the relationship between Pseudomonas aeruginosa（PA） and the apoptosis of U937 cell. Methods U937 cell apoptosis was induced by PA in groups of U937： PA= 1∶10,1∶20,1∶50 and 1∶100,respectively,and detected with Hochest 33258 fluorochrome staining and Annexin V/PI assay. Results When the ratio between cell and bacterium was 1∶10,the apoptotic cells were found in all the groups.Hoechst 33258 fluorochrome staining and Annexin V/ PI assay showed that the percentage of apoptotic U937 for（11.67±1.75）% and（11.68±2.58）%,and the percentage of apoptotic cells was raised with the increase of the concentration of PA.Conclusion PA can induce the U937 cells apoplosis in a dose-dependent manner.
作者 朱小敏 柴文戍
机构地区 辽宁医学院附属第一医院
出处 《中国病原生物学杂志》 CSCD 2008年第4期263-265,共3页 Journal of Pathogen Biology
关键词 铜绿假单胞菌 细胞凋亡 U937细胞 Pseudomonas aeruginosa apoptosis U937 cell
分类号 R378.991 [医药卫生—病原生物学]
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参考文献10

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共引文献3

同被引文献44

  • 1周立新,张秀平,方滨,李轶男,誉铁鸥,毛克江,苏艳玲.重症监护病房感染患者铜绿假单胞菌的分离与耐药性分析[J].中华医院感染学杂志,2004,14(7):807-808. 被引量：61
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  • 3李玲,熊盛道,徐永健,张珍祥.铜绿假单胞菌外膜蛋白D_2与耐药性的关系研究[J].中华微生物学和免疫学杂志,2005,25(3):189-189. 被引量：5
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引证文献5

二级引证文献23

中国病原生物学杂志
2008年 第4期

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