胰高血糖素样多肽-1对OLETF大鼠肺泡毛细血管基板的影响

Effects of glucagon like peptide-1 treatment on the alveolar capillary basal lamina in Otsuka Long-Evans Tokushima Fatty rats

目的:电镜观察并定量分析胰高血糖素样多肽-1(glucagon like peptide-1,GLP-1)对自发性2型糖尿病OLETF(Otsuka Long-Evans Tokushima Fatty)大鼠肺泡毛细血管基板的影响。方法:将OLETF大鼠分为未治疗的OLETF组及GLP-1治疗组(OLETF/G组),对照为LETO(Long-Evans Tokushima Otsuka)组大鼠。用透射电镜观察及形态计量学方法测量肺泡毛细血管基板的超微结构及厚度。结果:与LETO组大鼠相比,OLETF大鼠肺泡毛细血管内皮细胞基板与Ⅰ型肺泡上皮细胞基板融合部(F-BL)及肺泡毛细血管内皮细胞基板(Cap-BL)均增厚,分别为(110.60±14.14)nmvs(57.30±11.08)nm和(118.40±19.12)nmvs(66.80±8.63)nm(P<0.01)。OLETF/G组大鼠肺F-BL及Cap-BL均比OLETF组大鼠明显变薄,分别为(79.70±5.44)nmvs(110.60±14.14)nm和(69.80±3.32)nmvs(118.40±19.12)nm(P<0.01)。结论:OLETF大鼠肺泡毛细血管基板发生明显的超微结构变化;GLP-1治疗能减轻OLETF大鼠肺泡毛细血管基板损伤。

Objective： To observe and quantitatively analyze the effect of glucagon like peptide-1 （ GLP-1 ） on the alveolar capillary basal lamina in spontaneous type 2 diabetes mellitus animal model Otsuka Long-Evans Tokushima Fatty （OLETF） rats. Methods： Experimental rats were divided into three groups： OLETF group, GLP-1-treated group （OLETF/G group）, and Long-Evans Tokushima Otsuka group （ LETO group） as control. The ultrastructure and thickness of the alveolar capillary basal lamina in the rats were examined by transmission electron microscopy and morphometry methods. Results： The fused basal lamina （F-BL） of the alveolar capillary endothelium and type I epithelial basal lamina, and the alveolar capillary endothelium basal lamina （Cap-BL） were thickened in OLETF rats than those of LETO rats [ （ 110.60 ± 14.14） nm vs （57.30 ± 11.08） nm, and （ 118.40 ± 19.12） nm vs （66.80 ± 8.63 ） nm, P 〈 0.01 ]. F-BL and Cap-BL were thinned in the OLETF/G group as compared with OLETF group [（79.70 ±5.44） nm vs （110.60 ± 14. 14） nm and （69. 80 ±3. 32） nm vs （118.40 ± 19.12） nm, P 〈 0.01]. Conclusion： Our studies suggest the existence of ultrastructural changes of alveolar capillary basal lamina in OLETF rats. GLP-1 intervention decreases the damage of alveolar capillary basal lamina in OLETF rats.