血小板源性生长因子受体-α在胃肠道间质瘤表达的意义

Expression of Platelet-Derived Growth Factor Receptor-α in Gastrointestinal Stromal Tumors

目的探讨血小板源性生长因子受体(PDGFRα-)在胃肠道间质瘤中表达的意义。方法运用EnVision免疫组织化学染色检测CD117和PDGFRα-蛋白在94例胃肠道间质瘤中的表达。结果PDGFRα-的表达与胃肠道间质瘤发生的部位和组织学类型明显相关。26例呈中度以上的阳性染色,其中发生于胃24例,肠道1例,胃肠道外1例(P<0.01)。上皮细胞型和混合细胞型胃肠道间质瘤多呈中度以上弥漫性细胞膜或核旁点状阳性,梭形细胞型表达较弱,多呈局灶性(P<0.01)。PDGFR-α的表达与CD117的表达有关,24例CD117阴性或弱阳性病例中,13例(54.2%)PDGFRα-呈中度以上阳性;70例CD117中度以上阳性病例中,57例(81.4%)PDG-FRα-呈阴性或弱阳性(P<0.05)。结论PDGFRα-的表达与胃肠道间质瘤的发生部位、组织学类型和C-kit基因的表达有关,可能也是本病的重要发病机制之一。

Objective To explore the significance of platelet-derived growth factor receptor-a （PDGFR-a） expression in gastrointestinal stromal tumors（GIST）. Methods The expression of PDGFR a and CDl17 was detected by immunohistochemistry in 94 cases of GIST. Results The expression of PDGFR-a was relation to the location and histopathology of GIST. Of 26 cases with the moderate and strong positive, 24 cases were located in the stomach, 1 case in the small bowel, and the other in the extragastrointestinal（P〈0.01）. The expression of PDGFR-a in epithelioid cell type or epithelioid-spindle mixed cell type was exhibited moderate and strong positive staining which show membrance or a perinuclear dot-like pattern. Whereas the expression in the spindle cell type was weak or negative（P〈0. 01）. The expression of PDGFR-a correlated to the expression of CDl17. In 24 cases with the weak or negative expression of CDl17, 13 cases show the strong and moderate staining of PDGFR-a However in 70 cases with the expression strong or moderate of CDl17, 57 （81.4 %） exhibited the negative and weak expression of PDGFR-a（P〈0. 05）. Conclusion With regard to GIST, the expression of PDGFR-a is associated with the location, histopathology and the expression of CDl17. PDGFR-a may play a role in the pathogenesis of GIST.