大鼠大脑皮质神经元的原代培养

Primary Culture of the Rat Cortical Neurons

目的探讨大鼠大脑皮质神经元的培养方法。方法体外原代培养新生大鼠大脑皮质神经元,应用免疫细胞化学法及电子显微镜鉴定神经元。结果用神经基础培养基(Neurobasal-A)+B27培养的神经元纯度可达85%,生长状态较佳。结论以Neurobasal-A+B27培养的大鼠大脑皮质神经元活性较佳,纯度较高,是体外研究神经系统相关理论的良好模型。

Objective To observe the preemptive analgesic effect of pretreatment of flurbiprofen axetil on incisional pain in rats. Methods Animal model was made according to the Brennan method. Experimental rats were randomly divided into two main groups： P and C, each containing four subgroups, which were saline, flurbiprofen axetil 1, 3, 9 mg/kg, respectively. Group P received saline or flurbipro- fen axetil before incision and Group C 15 minutes after incision through tail vein. Calculative pain scorce test and hot plate test were performed at 2, 24 and 48 h. Results For both group P and C, flurbiprofen axetil（1, 3, 9 mg/kg） produced significant dose-dependent antinociception. The value of ED50 was 3.85 and 4. 20 mg/kg respectively. Confident intervals were （3.42, 4. 28） mg/kg and （3.96, 4. 44） mg/kg, respectively. Antinociception score and plantar withdrawl latency of group P were less than group C at 2, 24 and 48 h（P〈0.05）. Conclusion The administration of flurbiprofen axetilthrough tail vein can produce dose-dependent antinociception. Flurbiprofen axetil has showed preemptive analgesic effect.