饥饿和再投喂对翘嘴鲌幼鱼消化酶活性的影响

Effects of starvation and refeeding on activities of digestive enzymes in Culter alburnus Basilewsky juveniles

研究了饥饿和再投喂对翘嘴鲌（Culter alburnus Basilewsky）幼鱼前肠、后肠和肝胰脏消化酶活性的影响。在20．3～24．8℃条件下将375尾翘嘴鲌幼鱼[体质量（4．22±0．29）g]分为5组，分别饥饿0d（对照组）、4d、8d、12d和16d，饥饿后恢复投喂16d。结果显示，饥饿4d、8d后蛋白酶、淀粉酶和脂肪酶活性均显著下降；饥饿12d后，脂肪酶活性仍显著下降；随着饥饿时间延长，消化酶活性变化趋于平缓。恢复投喂后，蛋白酶活性迅速升高，其中肝胰脏蛋白酶活性（除饥饿4d组）升至显著高于饥饿前水平，然后逐渐恢复正常；恢复投喂4d后，除饥饿4d组后肠、肝胰脏及饥饿12d组前肠外，淀粉酶活性升至显著高于饥饿前水平，8d后恢复正常。与淀粉酶活性相似，脂肪酶活性先迅速升至显著高于饥饿前水平，然后再逐渐恢复正常，饥饿4d组的脂肪酶活性变幅较小，饥饿时间较长的，脂肪酶活性变幅较大。[中国水产科学，2008，15（3）：439-445]

The effects of starvation for diferent time and refeeding on the activities of digestive enzymes （protease, amylase and lipase） in three parts of digestive organs （foregut, hindgut and hepatopancreas） of Culter alburnus Basilewsky juveniles were investigated indoor under 20.3-24.8 ℃. After two weeks acclimation, 375 juveniles, body weight （4.22±0.29） g, were divided into five groups （A, B, C, D and E respectively, three replicates, each 25 individuals）, which were starved for 0 d （control）, 4 d, 8 d, 12 d and 16 d, respectively, then all re-fed for I6 d. The results showed that the activities of digestive enzymes were influenced markedly by starvation （P〈0.05）. The activities of three kinds of enzymes all decreased sharply during the first 4-8 days of starvation; the lipase activities of fish starved for 12 d still decreased and were greatly lower than those starved for 8 d （P〈0.05）, and then the digestive enzymes activities tended to decrease smoothly. Upon refeeding, the protease activities in the three parts rose quickly, reaching peak on the 4th day, of which the protease activities in hepatopancreas （except the fish starved for 4 d） were greatly higher than those before starvation, and then fell down to the level before starvation; after refeeding for 4 d, the amylase activities in three parts mostly reached the level that was greatly higher than those before starvation, and then recovered to the normal level after refeeding for 8 d; the lipase activities also rose to a peak after refeeding, and then recovered to the normal level; the longer time the fish starved, the higher the lipase activities rose. The distribution of the activities of digestive enzymes in three parts of digestive organs of C. alburnus juveniles were also discussed in this paper. [Journal of Fishery Sciences of China, 2008, 15 （3）： 439-445]